Pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in Chilo suppressalis.
Pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in Chilo suppressalis.
复制标题
信息素结合蛋白增强二化螟嗅觉受体对性信息素的敏感性
DOI：
10.1038/srep13093
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发表时间：
2015-08-27
影响因子：
4.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Chang H;Liu Y;Yang T;Pelosi P;Dong S;Wang G
飞蛾的性交流为模拟和研究昆虫的感官知觉提供了一个简化的场景。pbp（信息素结合蛋白）和pr（信息素受体）都参与性信息素的检测，但它们之间的相互作用在很大程度上仍然未知。在本研究中，我们测量了Chilo suppressalis的4个重组PBPs （csupppbp）与信息素成分和类似物的结合亲和力，并利用爪蟾卵母细胞表达系统对6个重组PBPs进行了表征。有趣的是，当在PBPs存在的情况下记录pr的反应时，我们在几种组合中测量到信号的急剧增加以及这种组合系统的灵敏度。此外，与单独使用PBPs或PRs相比，适当组合pr和PBPs的识别能力有所提高。除了进一步支持PBPs在信息素检测和识别中的作用外，我们的数据首次表明，PRs和PBPs的适当组合提高了PBPs或PRs单独的识别能力。用不同配对的PBPs和pr测量的各种反应表明嗅觉系统的复杂性，即使是相对简单的探测性信息素的任务，也利用了高度复杂的组合方法。
Sexual communication in moths offers a simplified scenario to model and investigate insect sensory perception. Both PBPs (pheromone-binding proteins) and PRs (pheromone receptors) are involved in the detection of sex pheromones, but the interplay between them still remains largely unknown. In this study, we have measured the binding affinities of the four recombinant PBPs of Chilo suppressalis (CsupPBPs) to pheromone components and analogs and characterized the six PRs using the Xenopus oocytes expression system. Interestingly, when the responses of PRs were recorded in the presence of PBPs, we measured in several combinations a dramatic increase in signals as well as in sensitivity of such combined systems. Furthermore, the discrimination ability of appropriate combinations of PRs and PBPs was improved compared with the performance of PBPs or PRs alone. Besides further supporting a role of PBPs in the pheromone detection and discrimination, our data shows for the first time that appropriate combinations of PRs and PBPs improved the discrimination ability of PBPs or PRs alone. The variety of responses measured with different pairing of PBPs and PRs indicates the complexity of the olfaction system, which, even for the relatively simple task of detecting sex pheromones, utilises a highly sophisticated combinatorial approach.