Monoclonal antibodies to sweet taste proteins: II. Development of two different immunoassays for thaumatin and monellin.
Monoclonal antibodies to sweet taste proteins: II. Development of two different immunoassays for thaumatin and monellin.
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甜味蛋白单克隆抗体：II。
DOI：
10.1089/hyb.1993.12.177
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Linthicum,DS
中科院分区：
文献类型：
--
作者：
Bodani,UC;Anchin,JM;Linthicum,DS
已经开发了两种不同类型的用于检测和定量具有强烈甜味特性的植物蛋白索马甜和莫内林的免疫测定法。建立了一种用于定量索马甜的串联酶联免疫吸附试验（ELISA），其中一种抗索马甜的单克隆抗体（mAb）（TM-1-D）用作固相“捕获”抗体，而第二种mAb抗体（TM-1-C）用生物素标记。获得了测定的标准曲线，相关系数为0.987;免疫测定的检测限为5 ng/ml。用单克隆抗体建立了莫内林的竞争性酶免疫分析法。检查了生物素化的莫内蛋白和已知莫内蛋白标准品与mAb 4.2E的竞争性结合;该测定的最低检测限为30 μg/ml。
Two different types of immunoassays for the detection and quantitation of thaumatin and monellin, plant proteins with intense sweet taste properties, have been developed. A tandem enzyme-linked immunosorbent assay (ELISA) was developed for the quantitation of thaumatin, in which one monoclonal antibody (mAb) raised against thaumatin (TM-1-D) was used as the solid-phase "capture" antibody, while the second mAb antibody (TM-1-C) was labeled with biotin. A standard curve for the assay was obtained and had a correlation coefficient of 0.987; the detection limit of the immunoassay was 5 ng/ml. Using a single anti-monellin mAb, a competitive enzyme immunoassay (EIA) was developed for monellin. The competitive binding of biotinylated monellin and known monellin standards with mAb 4.2E was examined; this assay had a minimum detection limit of 30 μg/ml.