CHANGES IN MORPHOLOGY AND CELL-WALL STRUCTURE THAT OCCUR DURING GROWTH OF VIBRIO SP NCTC4716 IN BATCH CULTURE
CHANGES IN MORPHOLOGY AND CELL-WALL STRUCTURE THAT OCCUR DURING GROWTH OF VIBRIO SP NCTC4716 IN BATCH CULTURE
复制标题
DOI：
10.1099/00221287-86-1-12
复制
发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
PARK, RWA
中科院分区：
文献类型：
--
作者：
BAKER, DA;PARK, RWA
当在各种培养基中分批培养时，Vibriosp.nctc4716显示出不同的形态序列。在指数生长期，微生物呈粗壮的几乎直的杆状，弯曲的杆状（弧菌属的特征）。在稳定相，在下降相中主要为球形。由于碳/能源的耗尽，生长停止后形成球体。它们是不可存活的，培养物的存活取决于在衰退阶段保留的少数杆状形式。这些球体似乎是由壁中存在的肽聚糖的降解而不是完全去除引起的。虽然球含有较少的核酸和低分子量的细胞质成分比杆，许多仍然拥有一个完整的细胞质膜。
When grown in batch culture in various mediaVibriosp.nctc4716 displayed a distinct sequence of morphological forms. Organisms occurred as stout, almost straight rods in exponential phase, curved rods (characteristic ofVibriospp.) in stationary phase, and predominantly as spheres in decline phase. The spheres were formed after growth had ceased due to the depletion of the carbon/energy source. They were not viable, survival of the culture depending on the few rod forms that remained during the decline phase. The spheres seemingly arose from degradation, but not complete removal, of the peptidoglycan present in the walls. Though spheres contained less nucleic acid and low molecular weight cytoplasmic constituents than did rods, many still possessed an intact cytoplasmic membrane.