THERMOTOLERANCE OF A LONG-LIVED MUTANT OF CAENORHABDITIS-ELEGANS
THERMOTOLERANCE OF A LONG-LIVED MUTANT OF CAENORHABDITIS-ELEGANS
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DOI：
10.1093/geronj/49.6.b270
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发表时间：
1994-11-01
期刊：
影响因子：
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通讯作者：
JOHNSON, TE
中科院分区：
文献类型：
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作者：
LITHGOW, GJ;WHITE, TM;JOHNSON, TE
秀丽隐杆线虫的同步队列在20摄氏度下生长，然后在30摄氏度或35摄氏度下应激。野生型和年龄-1突变株的内在耐热性进行了评估，通过测量后代生产或生存。除了寿命（Age）增加之外，Age-1突变导致内在耐热性（Itt）的高度显著增加，如通过在35 ℃下的存活所测量的。对于不育和非不育蠕虫，ge菌株的平均存活时间比非年龄菌株长约45%。耐热性下降的整个寿命的年龄和非年龄菌株，但在几乎所有年龄观察。未应激的1岁动物表现出一致和显着的生育力不足。短的热应力可导致年龄和非年龄基因型的后代产量急剧减少。年龄-1突变体表现出一个小的，但一致的耐热性增加，测量生育力。我们认为，年龄菌株科普环境压力的能力增强可能与其较低的年龄特定死亡率在机械上相关。
Age-synchronous cohorts of Caenorhabditis elegans were grown at 20 degrees C, then stressed at 30 degrees C or 35 degrees C. Intrinsic thermotolerance of wild type and age-1 mutant strains was assessed by measuring either progeny production or survival. in addition to increased life span (Age), mutation of age-1 results in a highly significant increased intrinsic thermotolerance (Itt) as measured by survival at 35 degrees C. Mean survival of ge strains is approximately 45% longer than that of non-Age strains for both sterile and nonsterile worms. Thermotolerance declines across the life span of both Age and non-Age strains, but In was observed at almost all ages. Unstressed age-1 animals showed a consistent and significant fertility deficit. Short thermal stresses can cause a dramatic reduction in progeny production for both Age and non-Age genotypes. Mutants of age-1 showed a small but consistent increased thermotolerance as measured by fertility. We propose that the enhanced ability of Age strains to cope with environmental stress may be mechanistically related to their lower age-specific mortality rates.