Almond Consumption for 8 Weeks Altered Host and Microbial Metabolism in Comparison to a Control Snack in Young Adults.
Almond Consumption for 8 Weeks Altered Host and Microbial Metabolism in Comparison to a Control Snack in Young Adults.
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DOI：
10.1080/07315724.2021.2025168
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发表时间：
2023-03-01
期刊：
影响因子：
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通讯作者：
Ortiz, Rudy M
中科院分区：
文献类型：
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作者：
Dhillon, Jaapna;Newman, John W;Ortiz, Rudy M
食用杏仁可以改善心脏代谢（CM）健康。然而，这些好处背后的机制并没有得到很好的描述。这项研究探讨了8周内食用杏仁和饼干零食对年轻人代谢谱变化的影响。（clinicaltrials.gov ID：NCT 03084003）。（n=73，年龄：18- 19岁，BMI：18- 41 kg/m2）随机分为两组，每组38只，每天食用杏仁（2 oz/d，n=38）和等热量控制零食全麦饼干（325 kcal/d，n=35），共8周。分别于干预前、干预后4周和8周采集血样。通过亲水相互作用色谱四极杆（Q）飞行时间（TOF）质谱（MS/MS）、气相色谱（GC）TOF MS、CSH-ESI（电喷雾）QTOF MS/MS和游离PUFA、总脂肪酸、氧脂素和内源性大麻素的靶向分析定量血清中的代谢物丰度。进行了基线调整的线性混合模型分析，并将这些结果用于富集和网络分析。利用PICRUST 2对粪便样品的16 SrRNA测序进行了微生物群落途径预测。杏仁消费丰富了不饱和甘油三酯、不饱和磷脂酰胆碱、饱和和不饱和溶血磷脂酰胆碱、三羧酸和生育酚簇（p
Almond consumption can improve cardiometabolic (CM) health. However, the mechanisms underlying those benefits are not well characterized. This study explored the effects of consuming a snack of almonds vs. crackers for 8weeks on changes in metabolomic profiles in young adults (clinicaltrials.gov ID: NCT03084003).Participants (n=73, age: 18-19years, BMI: 18-41kg/m2) were randomly assigned to consume either almonds (2oz/d, n=38) or an isocaloric control snack of graham crackers (325kcal/d, n=35) daily for 8weeks. Blood samples were collected at baseline prior to and at 4 and 8weeks after the intervention. Metabolite abundances in the serum were quantified by hydrophilic interaction chromatography quadrupole (Q) time-of-flight (TOF) mass spectrometry (MS/MS), gas chromatography (GC) TOF MS, CSH-ESI (electrospray) QTOF MS/MS, and targeted analyses for free PUFAs, total fatty acids, oxylipins and endocannabinoids. Linear mixed model analyses with baseline-adjustment were conducted, and those results were used for enrichment and network analyses. Microbial community pathway predictions from 16S rRNA sequencing of fecal samples was done using PICRUST2.Almond consumption enriched unsaturated triglycerides, unsaturated phosphatidylcholines, saturated and unsaturated lysophosphatidylcholines, tricarboxylic acids, and tocopherol clusters (p