Dynamic behavior of tea catechins interacting with lipid membranes as determined by NMR spectroscopy
Dynamic behavior of tea catechins interacting with lipid membranes as determined by NMR spectroscopy
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DOI：
10.1021/jf0712402
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发表时间：
2007-11-28
影响因子：
6.1
通讯作者：
Nakayama, Tsutomu
中科院分区：
文献类型：
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作者：
Uekusa, Yoshinori;Kamihira, Miya;Nakayama, Tsutomu
通过溶液核磁共振技术研究了茶儿茶素（例如表儿茶素没食子酸酯 (ECg) 和表没食子儿茶素没食子酸酯 (EGCg)）与各向同性 bicelle 模型脂质膜之间的相互作用。 H-1 NMR 测量提供了来自 ECg 和 EGCg 中 B 环和没食子酰基部分的信号，这些信号明显发生了偏移，并且在儿茶素与 bicelles 相互作用时，其质子 T、弛豫时间缩短。这些结果表明 B 环和没食子酰基部分在此相互作用中发挥重要作用。核奥沃豪塞效应光谱实验表明，B环和没食子酰基部分位于磷脂三甲基铵基团中的γ-H附近。基于这些发现，我们提出 ECg 和 EGCg 通过胆碱部分与脂膜表面相互作用。
Interaction between tea catechins, such as epicatechin gallate (ECg) and epigallocatechin gallate (EGCg), and isotropic bicelle model lipid membranes was investigated by solution NMR techniques. H-1 NMR measurements provided signals from the B-ring and the galloyl moiety in ECg and EGCg that were obviously shifted, and whose proton T, relaxation times were shortened upon interaction of the catechins with the bicelles. These results indicate that the B-ring and the galloyl moiety play an important role in this interaction. Nuclear Overhauser effect spectrometry experiments demonstrated that the B-ring and the galloyl moiety are located near the gamma-H in the phospholipid trimethylammonium group. On the basis of these findings, we propose that ECg and EGCg interact with the surface of lipid membranes via the choline moiety.