A quantitative proteomic analyses of primary myocardial cell injury induced by heat stress in chicken embryo.
A quantitative proteomic analyses of primary myocardial cell injury induced by heat stress in chicken embryo.
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DOI：
10.1016/j.jtherbio.2023.103461
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发表时间：
2023-01
影响因子：
2.7
通讯作者：
Rui Wang;Yan-li Guo;Zhaoguo Shi;Shizhen Qin
中科院分区：
文献类型：
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作者：
Rui Wang;Yan-li Guo;Zhaoguo Shi;Shizhen Qin
本研究建立鸡胚心肌细胞在42℃条件下热应激4 h的模型，采用DIA进行蛋白质组学分析，鉴定出245个差异表达蛋白（DEPs） （q值<0.05,fold change >1.5），其中63个蛋白表达上调，182个蛋白表达下调。许多与代谢、氧化应激、氧化磷酸化和细胞凋亡有关。基因本体（Gene Ontology， GO）分析表明，热应激下许多dep参与代谢产物和能量、细胞呼吸、催化活性和刺激的调节。京都基因与基因组百科全书（KEGG）分析显示，DEPs在代谢途径、氧化磷酸化、柠檬酸循环（TCA循环）、心肌收缩和碳代谢中富集。该结果有助于了解热应激对心肌细胞乃至心脏的影响及其在蛋白水平上可能的作用机制。
In this study, the model of heat stress was constructed in primary chick embryonic myocardial cells at 42 °C for 4 h. Proteome analysis using DIA identified 245 differentially expressed proteins (DEPs) (Q-value <0.05, fold change >1.5), of which 63 proteins were up-regulated and 182 proteins were down-regulated. Many were related to metabolism, oxidative stress, oxidative phosphorylation and apoptosis. Gene Ontology (GO) analysis showed that many DEPs under heat stress were involved in regulating metabolites and energy, cellular respiration, catalytic activity and stimulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEPs were enriched in metabolic pathways, oxidative phosphorylation, citrate cycle (TCA cycle), cardiac muscle contraction, and carbon metabolism. The results could help understanding of the effect of heat stress on myocardial cells and even the heart and possible action mechanism at the protein level.