Isolation and characterization of a gene cluster for dibenzofuran degradation in a new dibenzofuran-utilizing bacterium, Paenibacillus sp strain YK5
Isolation and characterization of a gene cluster for dibenzofuran degradation in a new dibenzofuran-utilizing bacterium, Paenibacillus sp strain YK5
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DOI：
10.1007/s00203-005-0045-9
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Kudo, T
中科院分区：
文献类型：
--
作者：
Iida, T;Nakamura, K;Kudo, T
孢子形成细菌菌株能够利用二苯并呋喃（DF）作为唯一的碳源和能源进行了分离。分离物的特征证明了它们被分类为类芽孢杆菌属，并且它们最近的亲戚是P. naphthalenovorans。利用芳烃双加氧酶α亚基（AhDOa）基因的简并引物和菌株YK 5的基因组DNA进行基因分离。PCR产物克隆的核苷酸序列分析表明，菌株YK 5携带至少5个不同的AhDOa基因。北方杂交分析表明，一个AhDOa基因的转录含有DF的培养条件下。分离编码AhDOa的基因簇。预测编码外二醇双加氧酶（dbfB）和水解酶（dbfC）的基因被发现是编码的α和β亚基的AhDO（dbfA 1和dbfA 2，分别）的基因的上游;后两个基因产物显示60和53%的同源性DbfA 1和DbfA 2的氨基酸序列的土杆菌属DBF 63，分别。用dbf基因簇转化的两个有效类芽孢杆菌JCM 9077菌株获得了将DF转化为2，2 '，3-三羟基联苯（THBP）和水杨酸（SAL）的能力。这些结果表明，在这项研究中分离的基因簇编码的酶参与DF代谢在YK 5。
Spore-forming bacterial strains capable of utilizing dibenzofuran (DF) as a sole source of carbon and energy were isolated. Characteristics of the isolates justified their classification into the genus Paenibacillus, and their closest relative was P. naphthalenovorans. Degenerate primers for aromatic hydrocarbon dioxygenase alpha subunit (AhDOa) genes and genomic DNA of the strain YK5 were used for gene isolation. The nucleotide sequences of clones of the PCR products revealed that the strain YK5 carries at least five different AhDOa genes. Northern hybridization analysis showed that one of the AhDOa genes was transcribed under DF-containing culture conditions. A gene cluster encoding the AhDOa was isolated. The genes predicted to encode extradiol dioxygenase (dbfB) and hydrolase (dbfC) were found to be an upstream of genes encoding the alpha and beta subunit of the AhDO (dbfA1 and dbfA2, respectively); the latter two gene products showed 60 and 53% identity to the amino acid sequences of DbfA1 and DbfA2 of Terrabacter sp. DBF63, respectively. Two Paenibacillus validus JCM 9077 strains transformed with the dbf gene clusters acquired the ability to convert DF to 2,2',3-trihydroxybiphenyl (THBP) and salicylic acid (SAL). These results suggest that the enzymes encoded by the gene cluster isolated in this study are involved in DF metabolism in YK5.