One-Pot Microwave Synthesis of Water-Dispersible, High Fluorescence Silicon Nanoparticles and Their Imaging Applications in Vitro and in Vivo
One-Pot Microwave Synthesis of Water-Dispersible, High Fluorescence Silicon Nanoparticles and Their Imaging Applications in Vitro and in Vivo
复制标题
一锅微波合成水分散性高荧光硅纳米颗粒及其体外和体内成像应用
DOI：
10.1021/acs.analchem.6b03209
复制
发表时间：
2016-12-06
影响因子：
7.4
通讯作者：
Zhang, Yu-Kui
中科院分区：
文献类型：
--
作者：
Ye, Hong-Li;Cai, Shi-Jiao;Zhang, Yu-Kui
硅纳米颗粒（SiNPs）已经报道通过微波辅助方法在高压下合成。然而，仍然缺乏关于在常压下通过微波辅助方法合成SiNPs的知识。在这里，我们开发了一种新的，简单的，一锅微波辅助法合成SiNPs（类似于4.2 nm）具有良好的水溶性在常压下，通过使用甘油作为溶剂。此外，甘油可能是负责的光致发光量子产率（PLQY）值高达47%的所得SiNP。与水溶液中制备的纳米颗粒相比，有机溶剂的使用可以提供更少的纳米颗粒表面缺陷，从而提高荧光效率。所制备的SiNPs同时具有明亮的蓝绿色荧光、长寿命（约12.8 ns）、明显的双光子吸收上转换发光、超强的光稳定性和良好的低毒性。作为一个令人满意的探针，合成的SiNPs成功地应用于人宫颈癌细胞系（HeLa）和斑马鱼的荧光成像。
Silicon nanoparticles (SiNPs) have been reported to be synthesized by microwave-assisted methods under high pressure. However, there is still a lack of knowledge about the synthesis of SiNPs via microwave-assisted methods under normal pressure. Here we developed a new, facile, one-pot microwave-assisted method for the synthesis SiNPs (similar to 4.2 nm) with excellent water solubility under normal pressure by employing glycerol as the solvent. Furthermore, glycerol might be responsible for the photoluminescence quantum yield (PLQY) value up to 47% for the resultant SiNPs. The use of organic solvent could afford less nanoparticle surface defects compared with those prepared in aqueous solution, thus improving the fluorescent efficiency. The as-prepared SiNPs simultaneously featured bright blue-green fluorescence, long lifetime (similar to 12.8 ns), obvious up-conversion luminescence originating from two photon absorption, superbly strong photostability, and favorable low toxicity. As a satisfactory probe, the as-synthesized SiNPs were successfully applied in fluorescence imaging of human cervical carcinoma cell lines (HeLa) and zebrafish.