Partitioning and localization of spin-labeled amantadine in lipid bilayers: an EPR study.
Partitioning and localization of spin-labeled amantadine in lipid bilayers: an EPR study.
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DOI：
10.1021/js970381n
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发表时间：
1998-10
影响因子：
3.8
通讯作者：
W. Subczynski;J. Wojas;V. Pezeshk;A. Pezeshk
中科院分区：
文献类型：
--
作者：
W. Subczynski;J. Wojas;V. Pezeshk;A. Pezeshk
EPR 用于研究自旋标记金刚烷胺 (AA-SL) 在本体疏水性烃溶剂、轻质石蜡油和水之间以及脂质膜疏水性烃链区域和水之间的分布。 AA-SL 分子可溶于所研究系统的疏水性和极性区域。结果表明，L-α-二肉豆蔻酰磷脂酰胆碱液相膜的烃区域与水之间的AA-SL分配系数远高于本体烃溶剂与水之间的分配系数。此外，研究了 AA-SL 在由 L-α-二肉豆蔻酰磷脂酰胆碱、L-α-二棕榈酰磷脂酰胆碱和 L-α-二硬脂酰磷脂酰胆碱制成的多层脂质体膜中的分配随温度的变化，表明这些膜的主要相变没有突然变化。从我们的数据中还可以清楚地看出，AA-SL 几乎可以以与流体相膜相同的分配方式渗透到凝胶相膜中。此外，研究表明，至少部分 AA-SL 分子深深埋藏在膜的烃链区域中。
EPR was used to study the distribution of the spin-labeled amantadine (AA-SL) between the bulk hydrophobic-hydrocarbon solvent, light paraffin oil, and water and between hydrophobic-hydrocarbon chain region of lipid membranes and water. The AA-SL molecules were soluble in both hydrophobic and polar regions of investigated systems. It was shown that the partition coefficient of AA-SL between the hydrocarbon region of the L-alpha-dimyristoylphosphatidylcholine fluid-phase membrane and water is much higher than between bulk hydrocarbon solvent and water. Furthermore, the partitioning of AA-SL into membranes of multilamellar liposomes made of L-alpha-dimyristoylphosphatidylcholine, L-alpha-dipalmitoylphosphatidylcholine, and L-alpha-distearoylphosphatidylcholine was studied as a function of temperature, indicating no abrupt change at the main phase transition of these membranes. It is also clear from our data that AA-SL can penetrate into the gel-phase membrane practically with the same partitioning as into the fluid-phase membrane. Furthermore, it was shown that at least part of the AA-SL molecule is deeply buried in the hydrocarbon chain region of the membrane.