Anti-inflammatory and gut microbiota modulatory effects of polysaccharides from Fuzhuan brick tea on colitis in mice induced by dextran sulfate sodium.
Anti-inflammatory and gut microbiota modulatory effects of polysaccharides from Fuzhuan brick tea on colitis in mice induced by dextran sulfate sodium.
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DOI：
10.1039/d1fo02702f
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发表时间：
2021-12
期刊：
影响因子：
6.1
通讯作者：
Ziqi Zeng;Z. Xie;Guijie Chen;Yi Sun;Xiaoxiong Zeng;Zhonghua Liu
中科院分区：
文献类型：
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作者：
Ziqi Zeng;Z. Xie;Guijie Chen;Yi Sun;Xiaoxiong Zeng;Zhonghua Liu
研究了茯苓砖茶粗多糖（CFBTPS）及其纯化组分（FBTPS-3）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的影响。CFBTPS和FBTPS-3均表现出肠道抗炎活性，包括恢复小鼠体重、结肠长度和固体粪便重量，并降低疾病活动指数评分。此外，脂质运载蛋白-2在结肠炎中的表达可显著降低。降低血清中炎性细胞因子（IL-6、IL-1 β、IFN-γ、TNF-α）和脂多糖水平，降低结肠组织中炎性相关mRNA的表达。CFBTPS和FBTPS-3均能增加紧密连接蛋白Occludin、Claudin-1和ZO-1的表达，促进肠屏障功能。对于肠道微生物群，DSS治疗导致双歧杆菌的异常增殖，而FBTPS-3可以在一定程度上恢复这种紊乱。此外，FBPTS-3对拟杆菌属、副苏氏菌属和柯林斯氏菌属等益生菌的生长也有促进作用。CFBTPS和FBTPS-3均能减轻结肠炎，且FBTPS-3的抗炎作用优于CFBTPS。
In this study, the effects of crude Fuzhuan brick tea polysaccharides (CFBTPS) and their purified fraction (FBTPS-3) on colitis induced by dextran sulfate sodium (DSS) in mice were investigated. Both CFBTPS and FBTPS-3 exhibited intestinal anti-inflammatory activities, including restoring body weight, colon length and solid fecal weight, and decreasing the disease activity index score in mice. Moreover, the expression of lipocalin-2 in colitis could be significantly reduced. The inflammatory cytokines (IL-6, IL-1β, IFN-γ and TNF-α) and lipopolysaccharides in the serum and the expression of inflammation-related mRNA in the colon tissue were decreased. Both CFBTPS and FBTPS-3 could increase tight junction proteins (Occludin, Claudin-1 and ZO-1), promoting the intestinal barrier function. For gut microbiota, DSS treatment resulted in abnormal proliferation of Bifidobacteria, while FBTPS-3 could restore this disorder to a certain extent. In addition, FBPTS-3 promoted the growth of probiotics such as Bacteroides, Parasutterella and Collinsella. Both CFBTPS and FBTPS-3 could attenuate colitis; what's more, FBTPS-3 exhibited a better anti-inflammatory effect than CFBTPS.