Opioid- and GABA(A)-receptors are co-expressed by neurons in rat brain.
Opioid- and GABA(A)-receptors are co-expressed by neurons in rat brain.
复制标题
阿片受体和 GABA(A) 受体由大鼠脑中的神经元共同表达。
DOI：
10.1097/00001756-200008210-00004
复制
发表时间：
2000
期刊：
影响因子：
1.7
通讯作者：
Wessendorf,MW
中科院分区：
文献类型：
--
作者：
Kalyuzhny,AE;Dooyema,J;Wessendorf,MW
Pharmacological data suggest that opioids exert their excitatory action in brain indirectly, by inhibiting release of the inhibitory neurotransmitter GABA. However, it is also possible that single neuron may interact with both opioids and GABA. In the present study, we investigated whether neurons in rat midbrain and medulla express both opioid and GABA A receptors. Coronal sections through rat brain were double-stained using antibodies against the alpha 1 subunit of GABA A receptor that were combined with antibodies either against the cloned muopioid receptor (MORI) or the cloned kappa-opioid receptor (KORI). Neurons double-labeled for GABA A receptors and either MORI or KORI were found in many brain regions including inferior colliculus, mesencephalic trigeminal nuclei, pontine reticular nuclei and raphe interpositus nucleus. Neurons double-labeled for GABA A and MORI were observed less frequently than those labeled for GABA A and KORI. Our findings provide anatomical evidence that GABAergic and opioidergic systems are closely linked and activity of the same neuron may be regulated directly by both GABA and opioids.
