Using the flowing afterglow of a plasma to inactivate Bacillus subtilis spores:: Influence of the operating conditions
Using the flowing afterglow of a plasma to inactivate Bacillus subtilis spores:: Influence of the operating conditions
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DOI：
10.1063/1.373792
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发表时间：
2000-07-15
影响因子：
3.2
通讯作者：
Yahia, L
中科院分区：
文献类型：
--
作者：
Moreau, S;Moisan, M;Yahia, L
微波放电的流动余辉可用于有效灭活细菌孢子。我们对这样一个系统的运行条件进行了参数研究，结果表明参与杀死孢子的物种是氧原子和紫外线（UV）光子。氧原子以及发射由流动余辉携带的光子的激发原子和分子可以在整个灭菌室中使用。典型的操作条件为：气体混合物2%O-2/98%N-2，压力范围1-7托，气体流量0.5-3slm。在余辉气体温度不超过 50 摄氏度的情况下，吸收微波功率为 100 W，可在 40 分钟内完全灭活 10(6) 个枯草芽孢杆菌孢子，这是热敏医疗设备的一个重要特征。本方案取决于到达待灭菌物体的所有部分的气流以及足够快地传送到那里的短寿命活性物质。在我们的操作条件下，紫外线发射强度决定了灭菌时间，因为始终有足够的氧原子可用于该过程。 (C) 2000 年美国物理研究所。 [S0021-8979(00)08914-3]。
The flowing afterglow of a microwave discharge can be used to efficiently inactivate bacterial spores. We have conducted a parametric study of the operating conditions of such a system, which shows that the species participating in the killing of spores are oxygen atoms and ultraviolet (UV) photons. The oxygen atoms and the excited atoms and molecules emitting the photons being carried by the flowing afterglow can be made available throughout the sterilization chamber. Typical operating conditions are: gas mixture 2%O-2/98%N-2, pressure range 1-7 Torr and gas flow 0.5-3 slm. Total inactivation of 10(6) B. subtilis spores is achieved within 40 min with 100 W absorbed microwave power, at afterglow gas temperatures not exceeding 50 degrees C, a feature of interest for heat sensitive medical devices. The present scheme depends on the gas flow reaching all parts of the objects to be sterilized and on the short-lived active species being transported there sufficiently rapid. Under our operating conditions, it is the UV emission intensity that sets the sterilization time as there are always more than sufficient oxygen atoms available for the process. (C) 2000 American Institute of Physics. [S0021-8979(00)08914-3].