Low-frequency electromagnetic fields promote hair follicles regeneration by injection a mixture of epidermal stem cells and dermal papilla cells
Low-frequency electromagnetic fields promote hair follicles regeneration by injection a mixture of epidermal stem cells and dermal papilla cells
复制标题
低频电磁场通过注射表皮干细胞和真皮乳头细胞的混合物促进毛囊再生
DOI：
10.1080/15368378.2020.1793165
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发表时间：
2020-08-01
影响因子：
1.7
通讯作者：
Zhang, Mingsheng
中科院分区：
文献类型：
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作者：
Li, Xinping;Ye, Yan;Zhang, Mingsheng
低频电磁场（EMF）对生物工程化毛囊生成的生物效应尚未完全阐明。本研究旨在评估低频电磁场对毛囊再生的治疗效果。在该实验中，表皮干细胞（ESCs）和毛乳头（DP）细胞被分离并培养扩增。然后将含有ESCs和DP细胞的混合物植入裸鼠的表皮层或真皮层。将这些小鼠随机分为对照组和电磁场组，7天或14天后，采集皮肤标本，通过H&E染色评估毛发再生或生物工程化皮肤的形成情况。将混合物注射到裸鼠表皮层14天后，H&E染色显示新毛发形成了正确的结构，包括毛母质、毛干、内根鞘、外根鞘和毛乳头。与对照组相比，电磁场组的毛囊以更高的密度长出。当混合物植入真皮层7天后，与对照组新毛囊的特征相比，H&E染色还显示，在电磁场暴露后，混合物诱导在生物工程化上皮层中形成4 - 6层类似表皮的结构，且毛囊样结构的密度更高。我们的研究结果表明，注射ESCs和DP细胞的混合物并结合电磁场暴露，有助于诱导毛囊再生以及形成具有毛囊样结构的生物工程化皮肤。
ABSTRACT The bioeffects of low-frequency electromagnetic fields (EMF) on a bio-engineered hair follicle generation had not been fully elucidated. This present study was designed to evaluat the therapeutically effective of low frequency EMF on hair follicles regeneration. In this experiment, epidermal stem cells (ESCs) and dermal papilla (DP) cells were isolated and culture-expanded. Then the mixture containing of ESCs and DP cells was implanted into the epidermal layer or corium layer of nude mice. Those mice were divided at random into the control group and EMF group, 7 days or 14 days later, the skin specimens were harvested to assess for hair regeneration or a bio-engineered skin formation using H&E staining. After injection of the mixture into the epidermal layer of nude mice for 14 days, H&E staining showed that the new hair formed the correct structure comprising hair matrix, hair shaft, and inner root sheath, outer root sheath, and DP. Comparing to the control, the hair follicles erupted at a higher density in the EMF group. When the mixture was implanted into the corium layer for 7 days, comparing with the characteristics of new hair follicles in the control group, H&E staining also showed the mixture induced to form 4 ~ 6 epidermal layers with a higher density of hair follicle like-structures in the bioengineered epithelial layers after EMF exposure. Our results suggested that the injection of a mixture of ESCs and DP cells in combination with EMF exposure facilitated the induction of hair follicle regeneration and a bioengineered skin formation with hair follicle-like structures.