Transplantation of Mesenchymal Stem Cells Exerts a Greater Long-Term Effect Than Bone Marrow Mononuclear Cells in a Chronic Myocardial Infarction Model in Rat
Transplantation of Mesenchymal Stem Cells Exerts a Greater Long-Term Effect Than Bone Marrow Mononuclear Cells in a Chronic Myocardial Infarction Model in Rat
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DOI：
10.3727/096368909x480323
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Prosper, Felipe
中科院分区：
文献类型：
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作者：
Mazo, Manuel;Jose Gavira, Juan;Prosper, Felipe
本研究的目的是评估间充质干细胞（MSC）移植在慢性心肌梗死（MI）大鼠模型中的长期效果，并与骨髓单核细胞（BM-MNC）移植的效果进行比较。 MI 诱导五周后，大鼠被分配接受心肌内注射 10(6) GFP 表达细胞（BM-MNC 或 MSC）或培养基作为对照。移植后 3 个月进行心脏功能（超声心动图和 F-18-FDG-microPET）和组织学研究，并在实验过程中（1 周和 2 周以及 1 个月和 3 个月）分析细胞命运。这项研究的主要结果是，BM 衍生人群 BM-MNC 和 MSC 均诱导 LVEF 持久（3 个月）改善（BM-MNC：26.61 +/- 2.01% 至 46.61 +/- 3.7%，p
The aim of this study is to assess the long-term effect of mesenchymal stem cells (MSC) transplantation in a rat model of chronic myocardial infarction (MI) in comparison with the effect of bone marrow mononuclear cells (BM-MNC) transplant. Five weeks after induction of MI, rats were allocated to receive intramyocardial injection of 10(6) GFP-expressing cells (BM-MNC or MSC) or medium as control. Heart function (echocardiography and F-18-FDG-microPET) and histological studies were performed 3 months after transplantation and cell fate was analyzed along the experiment (1 and 2 weeks and 1 and 3 months). The main findings of this study were that both BM-derived populations, BM-MNC and MSC, induced a long-lasting (3 months) improvement in LVEF (BM-MNC: 26.61 +/- 2.01% to 46.61 +/- 3.7%, p