Cloning of the rat and human mitochondrial branched chain aminotransferases (BCAT(m))
Cloning of the rat and human mitochondrial branched chain aminotransferases (BCAT(m))
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DOI：
10.1016/s0167-4838(97)00044-7
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发表时间：
1997-04-25
期刊：
影响因子：
--
通讯作者：
Hutson, SM
中科院分区：
文献类型：
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作者：
Bledsoe, RK;Dawson, PA;Hutson, SM
已分离出大鼠和人线粒体支链氨基转移酶（BCAT（m））cDNA，并显示其编码具有27个氨基酸的前序列的41.2和41.3 kDa的成熟蛋白质。当大鼠BCAT（m）在COS-1细胞中过表达时，该蛋白质表现出BCAT活性并正确加工线粒体靶向序列。一组啮齿动物-人类体细胞杂交体的基因组DNA的Southern印迹分析显示，人类BCAT基因位于染色体19上，人类胞质酶（BCAT（c））基因位于染色体12上。最后，命名为BCAT 1的胞质基因和BCAT 2的线粒体BCAT基因的建议。
The rat and human mitochondrial branched chain aminotransferase (BCAT(m)) cDNAs have been isolated and shown to encode mature proteins of 41.2 and 41.3 kDa with presequences of 27 amino acids. When rat BCAT(m) is overexpressed in COS-1 cells, the protein exhibits BCAT activity and correct processing of the mitochondrial targeting sequence. Southern blot analysis of genomic DNA from a panel of rodent-human somatic cell hybrids revealed that the human BCAT, gene resides on chromosome 19 and the human cytosolic enzyme (BCAT(c)) gene on chromosome 12. Finally, the nomenclature BCAT1 for the cytosolic gene and BCAT2 for the mitochondrial BCAT gene is proposed.