Seizure-like events in disinhibited ventral slices of adult rat hippocampus
Seizure-like events in disinhibited ventral slices of adult rat hippocampus
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DOI：
10.1152/jn.1999.82.5.2130
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发表时间：
1999-11-01
影响因子：
2.5
通讯作者：
Jefferys, JGR
中科院分区：
文献类型：
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作者：
Borck, C;Jefferys, JGR
在成年大鼠腹侧海马切片中体外研究了持续 2-90 秒的癫痫放电，其中用荷包牡丹碱甲碘化物（BMI，5-30 μM）和将钾（[K+](o)）提高到 5 mM 来急性阻断抑制作用。这些癫痫样事件 (SLE) 包括三个不同的阶段，此处称为初级、次级和三级爆发。主要突发持续 90-150 毫秒。次要爆发持续了另外 70-250 毫秒，包括一系列与主要爆发相同的去极化的短后放电。最后，一系列三次爆发以 5-10 Hz 的峰值频率开始，可持续 >1 分钟。腹侧海马切片显示出比背侧海马切片对 SLE 的易感性显着更高。 SLE 被证明对 α-氨基-3-羟基-5-甲基-4-异恶唑丙酸 (AMPA) 受体拮抗剂敏感。它们对 N-甲基-D-天冬氨酸 (NMDA) 受体拮抗剂不敏感； 50μl D-2-氨基-5-膦酰基戊酸(D-AP5)确实选择性地阻断了瞬时二次爆发。即使存在内嗅皮层，系统性红斑狼疮也仅限于海马体本身。内嗅爆发可能会持续
Epileptic discharges lasting 2-90 s, were studied in vitro in slices from the ventral hippocampus of adult rats, in which inhibition was blocked acutely with bicuculline methiodide (BMI, 5-30 mu M) and potassium ([K+](o)) raised to 5 mM. These seizure-like events (SLEs) comprised three distinct phases, called here primary, secondary, and tertiary bursts. Primary bursts lasted 90-150 ms. Secondary bursts lasted a further 70-250 ms, comprising a short series of afterdischarges riding on the same depolarization as the primary burst. Finally a train of tertiary bursts started with a peak frequency of 5-10 Hz and could last >1 min. Slices from the ventral hippocampus showed significantly higher susceptibility to SLEs than did dorsal slices. SLEs proved sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonists. They were insensitive to N-methyl-D-aspartate (NMDA) receptor antagonists; 50 mu l D-2-amino-5-phosphonopentanoic acid (D-AP5) did block the transient secondary bursts selectively. SLEs were restricted to the hippocampus proper even if the entorhinal cortex was present. Entorhinal bursts could last