Entamoeba histolytica:: Apoptosis induced in vitro by nitric oxide species
Entamoeba histolytica:: Apoptosis induced in vitro by nitric oxide species
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DOI：
10.1016/j.exppara.2007.01.011
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发表时间：
2007-07-01
影响因子：
2.1
通讯作者：
Tamayo, Ruy Perez
中科院分区：
文献类型：
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作者：
Ramos, Espiridion;Olivos-Garcia, Alfonso;Tamayo, Ruy Perez
在一些寄生虫如利什曼原虫、锥虫和滴虫中已经发现了细胞凋亡。这一现象在溶组织内阿米巴中尚未观察到。本工作分析了硝普钠、亚硝酸钠和硝酸钠(NOS)对体外培养的溶组埃希氏菌细胞凋亡的影响。原虫与一氧化氮合酶孵育1小时后，YOPRO-1检测显示细胞发生凋亡，细胞存活率为95%。TUNEL、DNA片段化和低ATP水平。Caspase抑制剂Z-VAD-FMK抑制细胞内总半胱氨酸蛋白酶活性(CPA)，但对细胞凋亡无影响。当用一氧化氮合酶处理时，一些阿米巴功能，如补体抵抗和溶血活性降低，但CPA和红细胞吞噬功能不变。在体外用NOS处理后，寄生虫在24小时内几乎完全死亡，但注射到仓鼠肝脏后不到6小时，寄生虫就消失了。这些结果表明，NOS在体外诱导了溶组织埃希氏菌的细胞凋亡，使它们无法在金黄地鼠肝脏中存活。(C)2007 Elsevier Inc.保留所有权利。
Apoptosis has been described in some parasites like Leishmania, Trypanosoma, and Trichomonas. This phenomenon has not been observed yet in Entamoeba histolytica. This work analyzed the in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (Nos) on E histolytica apoptosis. Parasites incubated for 1 hr with NOs revealed apoptosis 6 h later (95% viability), demonstrated by YOPRO-1. TUNEL, DNA fragmentation and low ATP levels. The caspase inhibitor Z-VAD-FMK inhibited total intracellular cysteine protease activity (CPA) but had no effect on apoptosis. When treated with NOs some amebic functions like complement resistance and hemolytic activity decreased but CPA and erythrophagocytosis remained unchanged. After treatment in vitro with NOs, parasite death was almost complete at 24 h: but when injected into hamster livers they disappeared in less than 6 h. These results show that apoptosis is induced in vitro by Nos in E. histolytica and renders them incapable of surviving in hamster's livers. (c) 2007 Elsevier Inc. All rights reserved.