Phospholipid Encapsulated Semiconducting Polymer Nanoparticles: Their Use in Cell Imaging and Protein Attachment
Phospholipid Encapsulated Semiconducting Polymer Nanoparticles: Their Use in Cell Imaging and Protein Attachment
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DOI：
10.1021/ja1002179
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发表时间：
2010-03-24
影响因子：
15
通讯作者：
Hughes, Mary
中科院分区：
文献类型：
--
作者：
Howes, Philip;Green, Mark;Hughes, Mary
采用溶剂蒸发法合成了半导体聚合物纳米球，并将其包裹在磷脂胶束中。使用了四种不同的共轭聚合物，产生了在可见光谱范围内发射的纳米颗粒的水分散体。该合成方法简单，易于重现，所得纳米颗粒溶液具有高的胶体稳定性。由于这些封装的SPN不含任何有毒物质，并显示出良好的光学性能，它们似乎是一个有前途的成像剂在生物医学和成像应用。将SPN用于简单的荧光成像实验，并显示在SH-SY 5 Y神经母细胞瘤和活HeLa细胞中的摄取。羧酸官能化的SPN也被合成和共轭牛血清白蛋白（BSA）的马来酰亚胺介导的化学，在使用共轭聚合物实现靶向成像的关键步骤。
Semiconducting polymer nanospheres (SPNs) have been synthesized and encapsulated in phospholipid micelles by a solvent evaporation technique. Four different conjugated polymers were used, yielding aqueous dispersions of nanoparticles which emit across the visible spectrum. The synthesis was simple and easily reproducible, and the resultant nanoparticle solutions exhibited high colloidal stability. As these encapsulated SPNs do not contain any toxic materials and show favorable optical properties, they appear to be a promising imaging agent in biomedical and imaging applications. The SPNs were used in simple fluorescence imaging experiments and showed uptake in SH-SY5Y neuroblastoma and live HeLa cells. Carboxylic acid functionalized SPNs were also synthesized and conjugated to bovine serum albumin (BSA) by carbodiimide-mediated chemistry, a key step in the realization of targeted imaging using conjugated polymers.