Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods
Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods
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DOI：
10.1021/nl062962v
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发表时间：
2007-04-01
期刊：
影响因子：
10.8
通讯作者：
Ben-Yakar, Adela
中科院分区：
文献类型：
--
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
我们证明了使用金纳米棒作为明亮的对比剂的双光子发光（TPL）成像的癌细胞在一个三维的组织模型下降到75 μ m深。在760 nm激发光下，来自金纳米棒标记的癌细胞的TPL强度比来自未标记的癌细胞的双光子自发荧光（TPAF）发射强度亮3个数量级。它们的强信号、抗光漂白性、化学稳定性、易于合成、简单的缀合化学和生物相容性使金纳米棒成为用于上皮癌的双光子成像的有吸引力的造影剂。
We demonstrate the use of gold nanorods as bright contrast agents for two-photon luminescence (TPL) imaging of cancer cells in a three-dimensional tissue phantom down to 75 mu m deep. The TPL intensity from gold-nanorod-labeled cancer cells is 3 orders of magnitude brighter than the two-photon autofluorescence (TPAF) emission intensity from unlabeled cancer cells at 760 nm excitation light. Their strong signal, resistance to photobleaching, chemical stability, ease of synthesis, simplicity of conjugation chemistry, and biocompatibility make gold nanorods an attractive contrast agent for two-photon imaging of epithelial cancer.