Effect of 'Chloroxynil' on Agrobacterium-mediated transformation efficiency of Lilium cv 'Manissa'
Effect of 'Chloroxynil' on Agrobacterium-mediated transformation efficiency of Lilium cv 'Manissa'
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DOI：
10.1016/j.scienta.2020.109404
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发表时间：
2020-09-20
影响因子：
4.3
通讯作者：
Okazaki, Keichii
中科院分区：
文献类型：
--
作者：
Abbasi, Hojatollah;Naderi, Roohangiz;Okazaki, Keichii
百合基因转化技术是一种有效的技术手段，可以培育出花色新颖、抗病性强的百合新品种。然而，在像百合这样的单子叶植物中使用这种技术是困难的。引入了一种新的酚类化合物"氯苯腈“来代替”乙酰丁香酮“，并被认为可以提高Mum的转化效率。结果表明，在接种培养基和共培养培养基中添加氯苯腈能显著提高转化效率。在4 μ M时，在将其转移到再生培养基后7天，氯氧腈介导的愈伤组织再生增加。100 μ M乙酰丁香酮的转化效率为6.6%，而4 μ M CX的转化效率为11.1%.最后，在4 μ M CX下成功地获得了11株卡那霉素抗性植株，并通过PCR和RT-PCR技术验证了基因转化植株。
As a powerful technique, gene transformation can produce new lily cultivars with specific characteristics such as new flower colors and resistance to pathogens. However, it is difficult to use this technique in monocot plants like lilies. A new phenolic compound called 'chloroxynil' has been introduced to replace 'acetosyringone' and is considered to improve transformation efficiency of Mum. Our results showed that the addition of chloroxynil into the inoculation and co-cultivation media significantly improved the transformation efficiency. At 4 mu M, chloroxynil-mediated callus regeneration increased 7 days after it was transferred to the regeneration medium. Transformation efficiency was 6.6 % at 100 mu M acetosyringone, while 11.1 % at 4 mu M CX. Finally, 11 kanamycin-resistant plants were successfully produced at 4 mu M CX, and gene transformation in the plantlets was validated by PCR and RT-PCR techniques.