Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein
Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein
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DOI：
10.1006/bbrc.2001.5271
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发表时间：
2001-07-27
影响因子：
3.1
通讯作者：
Huttner, WB
中科院分区：
文献类型：
--
作者：
Corbeil, D;Fargeas, CA;Huttner, WB
小鼠突出蛋白是一个新的膜糖蛋白家族的第一个特征成员。它具有典型的膜拓扑结构，具有五个跨膜段和两个大的糖基化细胞外环。在人类、鱼、蝇和蠕虫中已发现了主要的同源物和旁同源物。最近，一个编码小鼠突起蛋白的大鼠同源基因的cDNA序列被报道[Zhu et al . (2001) Biochem]。Biophys。[参考文献].公共科学。281,951-956]。令人惊讶的是，由于单个核苷酸的缺失改变了阅读框并引入了一个过早终止密码子，从该cDNA中预测的蛋白质将对应于c端截断的突起蛋白，只有四个跨膜片段。在这里，我们报告的证据与Zhu等人（2001）的报告相反。我们分离了一个没有移码突变的大鼠珥蛋白cDNA，证实大鼠珥蛋白与其他哺乳动物珥蛋白一样，是一个全长120 kda的五aspan膜糖蛋白，并且没有检测到任何c端截断的大鼠珥蛋白。(C) 2001学术出版社。
Mouse prominin is the first characterized member of a novel family of membrane glycoproteins. It displays characteristic membrane topology with five transmembrane segments and two large glycosylated extracellular loops. Prominin orthologues and paralogues have been identified in human, fish, fly, and worm. Recently, a cDNA sequence encoding the rat homologue of mouse prominin has been reported [Zhu et al (2001) Biochem. Biophys. Res. Commun. 281, 951-956]. Surprisingly, due to a single nucleotide deletion that shifts the reading frame and introduces a premature stop codon, the protein predicted from this cDNA would correspond to a C-terminally truncated form of prominin with only four transmembrane segments. Here we report evidence that is in contrast to the report of Zhu et al (2001). We isolated a rat prominin cDNA devoid of any frameshift mutation, demonstrate that rat prominin, like the other mammalian prominins, is a full-length 120-kDa pentaspan membrane glycoprotein, and have not been able to detect any C-terminally truncated form of rat prominin. (C) 2001 Academic Press.