ENZYMATIC IRON OXIDATION BY LEPTOTHRIX-DISCOPHORA - IDENTIFICATION OF AN IRON-OXIDIZING PROTEIN
ENZYMATIC IRON OXIDATION BY LEPTOTHRIX-DISCOPHORA - IDENTIFICATION OF AN IRON-OXIDIZING PROTEIN
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DOI：
10.1128/aem.58.2.450-454.1992
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发表时间：
1992-02-01
影响因子：
4.4
通讯作者：
DEVRINDDEJONG, EW
中科院分区：
文献类型：
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作者：
CORSTJENS, PLAM;DEVRIND, JPM;DEVRINDDEJONG, EW
从铁锰氧化细菌Leptothrix disophora SS-1的废液中鉴定出一种铁氧化因子。它似乎是一种蛋白质，表观分子量约为15万。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对废液进行分级后，可证明其活性。分离到一个自发突变株，该突变株既不分泌锰氧化活性，也不分泌铁氧化活性，而对其他蛋白的分泌似乎没有影响。虽然这两种金属氧化因子的排泄可能是相关的，但其他性质的差异表明，锰和铁的氧化代表了两种不同的途径。通过斑点印迹分析，确定了不同菌种对金属的氧化能力不同。圆盘藻对铁和锰都有氧化作用，而纳氏球藻只氧化铁和两种假单胞菌。只氧化了锰。
An iron-oxidizing factor was identified in the spent culture medium of the iron- and manganese-oxidizing bacterial strain Leptothrix discophora SS-1. It appeared to be a protein, with an apparent molecular weight of approximately 150,000. Its activity could be demonstrated after fractionation of the spent medium by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A spontaneous mutant of L. discophora SS-1 was isolated which excreted neither manganese- nor iron-oxidizing activity, whereas excretion of other proteins seemed to be unaffected. Although the excretion of both metal-oxidizing factors was probably linked, the difference in other properties suggests that manganese and iron oxidation represent two different pathways. With a dot-blot assay, it was established that different bacterial species have different metal-oxidizing capacities. Whereas L. discophora oxidized both iron and manganese, Sphaerotilus natans oxidized only iron and two Pseudomonas spp. oxidized only manganese.