Enhanced antibody production in mice to the malaria antigen AMA 1 by CPG 7909 requires physical association of CpG and antigen
Enhanced antibody production in mice to the malaria antigen AMA 1 by CPG 7909 requires physical association of CpG and antigen
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DOI：
10.1016/j.vaccine.2007.05.007
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发表时间：
2007-07-20
期刊：
影响因子：
5.5
通讯作者：
Saul, Allan
中科院分区：
文献类型：
--
作者：
Mullen, Gregory E. D.;Aebig, Joan A.;Saul, Allan
CpG寡脱氧核苷酸是有效的免疫刺激剂。在这项研究中，CPG 7909与吸附在Alhydrogel（氢氧化铝）上的重组恶性疟原虫蛋白AMA 1-C1一起配制，并用于免疫小鼠。在单独的相同部位注射至AMA 1-C1/Alhydrogel中接受游离CPG 7909的小鼠具有与单独接受AMA 1-C1/Alhydrogel的小鼠相同的抗体应答。对于用与AMA 1-C1/Alhydrogel制剂结合的CPG 7909免疫的小鼠，存在钟形的CPG 7909剂量-反应曲线，其中最高抗体反应与饱和结合Alhydrogel的CPG 7909的浓度一致。在74%未结合的较高CPG 7909剂量下，与单独的AMA 1-C1/Alhydrogel相比，反应没有增强。我们的研究结果表明，吸附到铝凝胶时，CpG的佐剂效果是最佳的，并强调需要仔细表征疫苗制剂。(c)2007爱思唯尔有限公司保留所有权利。
CpG oligodeoxynucleotides are potent immunostimulants. In this study, CPG 7909 was formulated with the recombinant Plasmodium falciparum protein AMA1-C1 adsorbed to Alhydrogel (aluminum hydroxide) and used to immunize mice. Mice receiving free CPG 7909 in a separate same site injection to the AMA1-C1/Alhydrogel had the same antibody responses as mice receiving AMA1 -C1/Alhydrogel alone. For mice immunized with CPG 7909 bound to the AMA1-C1/Alhydrogel formulation, there was a bell shaped CPG 7909 dose-response curve with the highest antibody response co-incident with the concentration of CPG 7909 that saturated binding to the Alhydrogel. At a higher CPG 7909 dose where 74% was unbound, there was no enhancement of response over AMA1-C1/Alhydrogel alone. Our results suggest that the adjuvant effects of CpGs are optimal when adsorbed to Alhydrogel and highlight the need for careful characterization of the vaccine formulation. (c) 2007 Elsevier Ltd. All rights reserved.