Pupil diameter is not an accurate real-time readout of locus coeruleus activity.
Pupil diameter is not an accurate real-time readout of locus coeruleus activity.
复制标题
作者：
Megemont M;McBurney-Lin J;Yang H
瞳孔直径通常被认为是蓝斑(LC)活动的无创性读数。然而，它能多准确地被用来索引LC活性尚不清楚。为了解决这个问题，我们在小鼠中建立了瞳孔大小变化和LC尖峰活动之间的分级关系，其中瞳孔扩张随着LC尖峰数量的增加而单调增加。然而，这种关系存在着很大的变异性，以至于瞳孔直径只能用来准确地预测LC活动的一小部分。此外，在LC中，对于相同的光刺激，瞳孔表现出较大的节间波动。瞳孔-LC关系的变化与决策偏差相关的行为变量密切相关。总之，我们的数据表明，在瞳孔直径和LC活动之间的总体分级关系中存在显著的变异性，并进一步表明，瞳孔-LC关系受到大脑状态的动态调节，支持和扩展了我们之前的发现(Yang等人，2021)。
Pupil diameter is often treated as a noninvasive readout of activity in the locus coeruleus (LC). However, how accurately it can be used to index LC activity is not known. To address this question, we established a graded relationship between pupil size changes and LC spiking activity in mice, where pupil dilation increased monotonically with the number of LC spikes. However, this relationship exists with substantial variability such that pupil diameter can only be used to accurately predict a small fraction of LC activity on a moment-by-moment basis. In addition, pupil exhibited large session-to-session fluctuations in response to identical optical stimulation in the LC. The variations in the pupil–LC relationship were strongly correlated with decision bias-related behavioral variables. Together, our data show that substantial variability exists in an overall graded relationship between pupil diameter and LC activity, and further suggest that the pupil–LC relationship is dynamically modulated by brain states, supporting and extending our previous findings (Yang et al., 2021).