Induction of dermal fibroblasts into dermal papilla cell-like cells in hydrogel microcapsules for enhanced hair follicle regeneration
Induction of dermal fibroblasts into dermal papilla cell-like cells in hydrogel microcapsules for enhanced hair follicle regeneration
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DOI：
10.1016/j.apmt.2020.100805
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发表时间：
2020-12
影响因子：
8.3
通讯作者：
Bei Xie;Mengting Chen;Pinghui Ding;L. Lei;Xing Zhang;Dan Zhu;Yujian Zou;Z. Deng;Guoming Sun;Ji Li;H. Nie
中科院分区：
文献类型：
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作者：
Bei Xie;Mengting Chen;Pinghui Ding;L. Lei;Xing Zhang;Dan Zhu;Yujian Zou;Z. Deng;Guoming Sun;Ji Li;H. Nie
目前的方法无法获得足够的功能性人毛乳头细胞（DPC）来再生毛囊（HF）。本研究采用海藻酸钠-聚赖氨酸-海藻酸钠（阿帕）微囊化技术诱导人皮肤成纤维细胞（hDF）分化为hDPC样细胞（hDF-DPC）。我们的研究结果表明，衍生的hDF-DPC在分子水平上与原代DPC相当，它们可以诱导裸鼠毛囊样结构的形成和毛发色素沉着。RNA-seq分析表明在hDF-DPC转化期间Wnt信号传导途径的活化。同样，我们证明了小鼠DF在水凝胶微胶囊内分化成小鼠DPC样细胞。通过仿生微囊化将真皮成纤维细胞诱导成DPC样细胞，没有化学或基因重编程，将提供替代的、丰富的和安全的DPC来源，这将有助于绕过基于DPC的HF再生的瓶颈。
Current approaches fail at obtaining adequate functional human dermal papilla cells (DPCs) to regenerate hair follicles (HFs). Here we induce human dermal fibroblasts (hDFs) into hDPC-like cells (hDF-DPCs) through alginate-poly-L-lysine-alginate (APA) microencapsulation. Our results show that the derived hDF-DPCs are comparable to primary DPCs on the molecular level, and they can induce the formation of hair follicle-like structures and hair pigmentation in nude mice. RNA-seq analyses indicate the activation of Wnt signaling pathway during the hDF-DPCs conversion. Likewise, we demonstrate that mouse DFs differentiate into mouse DPC-like cells within the hydrogel microcapsules. The induction of dermal fibroblasts into DPC-like cells through biomimetic microencapsulation, devoid of chemical or gene reprogramming, would provide an alternative, abundant, and safe source of DPCs, which would help circumvent the bottleneck in DPC-based HF regeneration.