Characterization of the complete genome of Barley yellow striate mosaic virus reveals a nested gene encoding a small hydrophobic protein
Characterization of the complete genome of Barley yellow striate mosaic virus reveals a nested gene encoding a small hydrophobic protein
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大麦黄纹花叶病毒完整基因组的表征揭示了编码小疏水蛋白的嵌套基因
DOI：
10.1016/j.virol.2014.12.042
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xian-Bing Wang
中科院分区：
文献类型：
--
作者：
Teng Yan;Jing-Rong Zhu;Dianping Di;Qiang Gao;Yongliang Zhang;Aihong Zhang;Chong Yan;Hongqin Miao;Xian-Bing Wang
大麦黄纹花叶病毒（Barley yellow striate mosaic virus，BYSMV）是细胞弹状病毒属（Cytorhabdovirus）的成员之一，在农业上造成严重的损失.在此，我们克隆了BYSMV来源的小干扰RNA（siRNA），组装siRNA并使用RT-PCR重建BYSMV基因组。基因组由12，706个核苷酸组成，编码来自反基因组链的10个预测基因。BYSMV的主要结构蛋白与北方禾谷类花叶病毒（NCMV）的结构蛋白具有35%~ 62%的同源性。一个显着的区别是，BYSMV包含三个转录单位之间的P和M基因相比，在相应区域的NCMV的四个单位。出乎意料的是，在这个区域的中间mRNA编码基因5嵌套在一个替代框架内基因4通过泄漏扫描机制。基因5编码一种靶向内质网（ER）的小疏水蛋白。据我们所知，这是首次在植物弹状病毒中发现巢状基因。
Barley yellow striate mosaic virus(BYSMV), a member of the genusCytorhabdovirus, causes serious crop losses in agriculture. Here, we have cloned the BYSMV-derived small interfering RNAs (siRNAs), assembled the siRNAs and used RT-PCR to reconstruct the BYSMV genome. The genome consists of 12,706 nucleotides and encodes ten predicted genes from the antigenomic strand. The major BYSMV structural proteins share identities ranging from 35% to 62% with northern cereal mosaic virus (NCMV) counterparts. A notable difference is that BYSMV contains three transcriptional units residing between the P and M genes compared with four units in the corresponding region of NCMV. Unexpectedly, the middle mRNA in this region encodes gene5 nested in an alternative frame within gene4viaa leaky scanning mechanism. The gene5 encodes a small hydrophobic protein targeting to the endoplasmic reticulum (ER). To our knowledge, this is the first report of nested gene in plant rhabdoviruses.