Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats.
Eff ects of hemin, a heme oxygenase-1 inducer in L-arginine-induced acute pancreatitis and associated lung injury in adult male albino rats.
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DOI：
10.1515/enr-2017-0003
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Rifaai, R A
中科院分区：
文献类型：
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作者：
Aziz, N M;Kamel, M Y;Rifaai, R A
目的：本研究的目的是评估血红素氧合酶-1（HO-1）诱导剂氯化血红素对L-精氨酸诱导的大鼠急性胰腺炎的保护作用。急性胰腺炎（AP）被认为是一种严重影响患者健康的炎症性疾病。方法：将24只成年雄性白化病大鼠随机分为4组：对照组、急性胰腺炎（AP）组、AP前氯化血红素组和AP后氯化血红素组。诱导AP前给予氯化血红素可显著减弱L-精氨酸-诱导的胰腺炎和相关的肺部并发症，其特征在于与对照组相比，血清淀粉酶、脂肪酶、肿瘤坏死因子-α、一氧化氮水平升高，以及胰腺和肺部的组织结构变化。此外，用氯化血红素预处理显著补偿了总抗氧化能力的不足，并降低了AP观察到的升高的丙二醛水平。另一方面，后氯化血红素管理没有显示任何保护L-精氨酸诱导AP。结论：目前的研究表明，诱导HO-1氯化血红素预处理显着改善L-精氨酸诱导胰腺炎和相关的肺部并发症可能是由于其抗炎和抗氧化特性。
OBJECTIVE: The aim of the current study was to assess the protective outcome of hemin, a heme oxygenase-1 (HO-1) inducer on L-arginine-induced acute pancreatitis in rats. Acute pancreatitis (AP) is considered to be a critical inflammatory disorder with a major impact on the patient health. Various theories have been recommended regarding the pathophysiology of AP and associated pulmonary complications.METHODS: Twenty-four adult male albino rats were randomly divided into four groups: control group, acute pancreatitis (AP), hemin pre-treated AP group, and hemin post-treated AP group.RESULTS: Administration of hemin before induction of AP significantly attenuated the L-arginine- induced pancreatitis and associated pulmonary complications characterized by the increasing serum levels of amylase, lipase, tumor necrosis factor-alpha, nitric oxide, and histo-architectural changes in pancreas and lungs as compared to control group. Additionally, pre-treatment with hemin significantly compensated the deficits in total antioxidant capacities and lowered the elevated malondialdehyde levels observed with AP. On the other hand, post-hemin administration did not show any protection against L-arginine-induced AP.CONCLUSIONS: The current study indicates that the induction of HO-1 by hemin pre-treatment significantly ameliorated the L-arginine-induced pancreatitis and associated pulmonary complications may be due to its anti-inflammatory and antioxidant properties.