Resilience of Oocyte Germinal Vesicles to Microwave-Assisted Drying in the Domestic Cat Model
Resilience of Oocyte Germinal Vesicles to Microwave-Assisted Drying in the Domestic Cat Model
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DOI：
10.1089/bio.2014.0078
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发表时间：
2015-06-01
影响因子：
1.6
通讯作者：
Comizzoli, Pierre
中科院分区：
文献类型：
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作者：
Elliott, Gloria D.;Lee, Pei-Chih;Comizzoli, Pierre
将猫生发泡(GV)致密并注入受体细胞质的能力使我们能够探索一种新的育性保存策略，避免整个卵子冷冻。本研究的目的是了解失水和储存时间对GV DNA完整性的影响。未成熟的猫卵母细胞在1.5M海藻糖中暴露10min，然后在微波辅助下脱水0、5、10、15、20、25、30或40min。然后对卵母细胞进行再水化，以评估染色质构型和DNA碎裂的发生率(TUNEL法)。40min后水分含量逐渐降低(p0.05)。随着时间的推移，染色质的构型没有受到影响(p>；0.05)。DNA断裂的GV百分率在处理后0~30min(6.1%~12%)变化不大(p>；0.05)，但增加(p<0.05)。
The ability to compact and inject the cat germinal vesicle (GV) into a recipient cytoplast allows exploration of a new fertility preservation strategy that avoids whole oocyte freezing. The objective of the present study was to understand the impact of water loss and storage time on GV DNA integrity. Immature cat oocytes were exposed to 1.5M trehalose for 10min before microwave-assisted dehydration for 0, 5, 10, 15, 20, 25, 30, or 40min. Oocytes then were rehydrated to assess chromatin configuration and the incidence of DNA fragmentation (TUNEL assay). The moisture content progressively decreased (p0.05) after 40min. Chromatin configuration was unaffected (p>0.05) over time. The percentage of GVs with DNA fragmentation was unaltered (p>0.05) from 0 to 30min of treatment (range, 6.1%-12%), but increased (p