Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis.
Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis.
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DOI：
10.1038/85471
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发表时间：
2001-03-01
期刊：
影响因子：
82.9
通讯作者：
F채ssler, R
中科院分区：
文献类型：
--
作者：
Sakai, T;Johnson, KJ;F채ssler, R
纤维连接蛋白在胚胎发育中起重要作用，并在组织修复过程中显著表达。已经鉴定了两种形式的纤连蛋白：血浆纤连蛋白（pFn），其由肝细胞表达并以可溶性形式分泌到血浆中;和细胞纤连蛋白（cFn），其由成纤维细胞和其他细胞类型局部表达并沉积和组装到细胞外基质中的不溶性形式。为了研究pFn在体内的作用，我们使用Cre-loxP条件基因敲除技术产生pFn缺陷的成年小鼠。在这里，我们表明，pfn缺陷小鼠表现出增加的神经细胞凋亡和更大的梗死面积后短暂局灶性脑缺血。然而，pFn对皮肤伤口愈合和止血不利。
Fibronectin performs essential roles in embryonic development and is prominently expressed during tissue repair. Two forms of fibronectin have been Identified: plasma fibronectin (pFn), which Is expressed by hepatocytes and secreted In soluble form into plasma; and cellular fibronectin (cFn), an insoluble form expressed locally by fibroblasts and other cell types and deposited and assembled into the extracellular matrix. To investigate the role of pFn in vivo, we generated pfn-deficient adult mice using Cre-loxP conditional gene-knockout technology. Here we show that pfn-deficient mice show increased neuronal apoptosis and larger Infarction areas following transient focal cerebral ischemia. However, pFn is dispensable for skin-wound healing and hemostasis.