Cross-linked type I and type II collagenous matrices for the repair of full-thickness articular cartilage defects - A study in rabbits
Cross-linked type I and type II collagenous matrices for the repair of full-thickness articular cartilage defects - A study in rabbits
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DOI：
10.1016/s0142-9612(03)00143-1
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
van Kuppevelt, TH
中科院分区：
文献类型：
--
作者：
Buma, P;Pieper, JS;van Kuppevelt, TH
胶原基质的物理化学性质可能决定了插入全层关节软骨缺损后的组织反应。在本研究中，交联的I型和II型胶原基质，有和没有附着硫酸软骨素。植入青春期家兔股骨滑车全层缺损。采用一般组织学和两种半定量组织学分级系统评估植入后4周和12周的组织反应。植入4周后，I型胶原基质被软骨样组织完全填充。相比之下，II型胶原基质主要显示软骨组织仅在表面区和基质与软骨下骨的界面处。使大面积的基质失去组织。硫酸软骨素的附着似乎可以促进两种胶原基质的细胞长入和软骨组织的形成。植入12周后，基质之间的差异不太明显。软骨下缺损的深层部分大部分被新骨取代，并伴有基质降解。用纤维软骨组织修复I型胶原基基质缺损的原始软骨轮廓。含有II型基质的缺陷显示表面软骨样组织的数量增加。然而，并非所有动物的原始轮廓都能完全恢复，偶尔会留下中央凹陷或裂缝。结论：不同类型的胶原基质在全层关节软骨缺损中诱导不同的组织反应。I型胶原基质在引导软骨下起源的祖细胞进入缺损方面具有优势。在II型胶原基质中，细胞迁移较少，但入侵细胞被引导成软骨细胞表型。基于这些观察结果，我们认为由深层I型胶原蛋白和较浅层II型胶原蛋白组成的复合基质可能是软骨再生的首选基质。2003爱思唯尔科学有限公司版权所有。
The physico-chemical properties of collagenous matrices may determine the tissue response after insertion into full-thickness articular cartilage defects. In this study, cross-linked type I and type II collagen matrices, with and without attached chondroitin sulfate. were implanted into full-thickness defects in the femoral trochlea of adolescent rabbits. The tissue response was evaluated 4 and 12 weeks after implantation by general histology and two semi-quantitative histological grading systems.Four weeks after implantation, type I collagenous matrices were completely filled with cartilage-like tissue. By contrast, type II collagenous matrices revealed predominantly cartilaginous tissue only at the superficial zone and at the interface of the matrix with the subchondral bone. leaving large areas of the matrix devoid of tissue. Attachment of chondroitin sulfate appeared to promote cellular ingrowth and cartilaginous tissue formation in both types of collagen matrices.Twelve weeks after implantation, the differences between the matrices were less pronounced. The deep parts of tile subchondral defects were largely replaced by new bone with a concomitant degradation of the matrices. The original cartilage contours in defects with type I collagen-based matrices were repaired with fibro-cartilaginous tissue. Defects containing type II matrices showed an increase in the amount of superficial cartilage-like tissue. The original contour, however, was not completely restored in all animals, occasionally leaving a central depression or fissure.It is concluded that different types of collagen matrices induce different tissue responses in full-thickness articular cartilage defects. Type I collagen-based matrices are superior to guide progenitor cells from a subchondral origin into the defect. In type II collagen-based matrices cell migration is less, but invading cells are directed into a chondrocyte phenotype. Based on these observations it is suggested that a composite matrix consisting of a deep layer of type I collagen and a more superficial layer of type II collagen may be the matrix of choice for cartilage regeneration. (C) 2003 Elsevier Science Ltd. All rights reserved.