Ribosome A and P sites revealed by length analysis of ribosome profiling data.
Ribosome A and P sites revealed by length analysis of ribosome profiling data.
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DOI：
10.1093/nar/gkv200
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发表时间：
2015-04-20
影响因子：
14.9
通讯作者：
Hilser VJ
中科院分区：
文献类型：
--
作者：
Martens AT;Taylor J;Hilser VJ
The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and has furthered investigations into the connections between codon biases and translation rates. Yet the location of the codon being decoded in ribosome footprints is still unknown, and has been complicated by the recent observation of footprints with non-canonical lengths. Here we show how taking into account the variations in ribosome footprint lengths can reveal the ribosome aminoacyl (A) and peptidyl (P) site locations. These location assignments are in agreement with the proposed mechanisms for various ribosome pauses and further enhance the resolution of the profiling data. We also show that GC-rich motifs at the 5′ ends of footprints are found in yeast, calling into question the anti-Shine-Dalgarno effect's role in ribosome pausing.
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影响因子：
14.9
作者：
Hansen KD;Brenner SE;Dudoit S
通讯作者：
Dudoit S
影响因子：
5.6
作者：
Spencer, Paige S.;Siller, Efrain;Anderson, John F.;Barral, Jose M.
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Barral, Jose M.
影响因子：
5.6
作者：
Siller, Efrain;DeZwaan, Diane C.;Barral, Jose M.
通讯作者：
Barral, Jose M.
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Lareau LF;Hite DH;Hogan GJ;Brown PO
通讯作者：
Brown PO
影响因子：
3.2
作者：
SCHLEIF, R;HESS, W;ELLIS, D
通讯作者：
ELLIS, D