Assembly of D-alanyl-lipoteichoic acid in Lactobacillus casei: mutants deficient in the D-alanyl ester content of this amphiphile.
Assembly of D-alanyl-lipoteichoic acid in Lactobacillus casei: mutants deficient in the D-alanyl ester content of this amphiphile.
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干酪乳杆菌中 D-丙氨酰-脂磷壁酸的组装：该两亲物的 D-丙氨酰酯含量不足的突变体。
DOI：
10.1128/jb.169.4.1702-1711.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Neuhaus,FC
中科院分区：
文献类型：
--
作者：
Ntamere,AS;Taron,DJ;Neuhaus,FC
来自干酪乳杆菌ATCC 7469的d -丙烯酰脂磷胆酸（D-alanyl- lta）含有与d -丙烯酰酯残基酰化的聚甘油磷酸段。这些残基的生理功能尚不清楚。分离并鉴定了五种缺乏这种两亲体酯的突变菌株。与亲本相比，菌株AN-1和AN-4将不到10%的D-[14C]丙氨酸带入LTA，而菌株AN-2、AN-3和AN-5将50%的D-[14C]丙氨酸带入LTA。D-[14C]alanyl-亲脂LTA的合成在第一组中几乎不存在，在第二组中约为30%。突变菌株合成并选择了用于LTA组装的糖脂锚点。此外，所有菌株合成LTA的聚甘油磷酸酯部分的程度与亲本相同或更高。结果表明，突变菌株AN-1和AN-4的膜在受体LTA存在的情况下，对LTA的d -丙烯酰化存在缺陷。突变株AN-2和AN-3似乎部分缺乏d -丙氨酸激活酶的量。形态异常和细胞分离缺陷似乎是由于d -丙烯酰酯含量不足造成的。
D-Alanyl-lipoteichoic acid (D-alanyl-LTA) from Lactobacillus casei ATCC 7469 contains a poly(glycerophosphate) moiety that is acylated with D-alanyl ester residues. The physiological function of these residues is not well understood. Five mutant strains of this organism that are deficient in the esters of this amphiphile were isolated and characterized. When compared with the parent, strains AN-1 and AN-4 incorporated less than 10% of D-[14C]alanine into LTA, whereas AN-2, AN-3, and AN-5 incorporated 50%. The synthesis of D-[14C]alanyl-lipophilic LTA was virtually absent in the first group and was approximately 30% in the second group. The mutant strains synthesized and selected the glycolipid anchor for LTA assembly. In addition, all of the strains synthesized the poly(glycerophosphate) moiety of LTA to the same extent as did the parent or to a greater extent. It was concluded that the membranes from the mutant strains AN-1 and AN-4 are defective for D-alanylation of LTA even though acceptor LTA is present. Mutant strains AN-2 and AN-3 appear to be partially deficient in the amount of the D-alanine-activating enzyme. Aberrant morphology and defective cell separation appear to result from this deficiency in D-alanyl ester content.