Essential roles of integrin-mediated signaling for the enhancement of malignant properties of melanomas based on the expression of GD3
Essential roles of integrin-mediated signaling for the enhancement of malignant properties of melanomas based on the expression of GD3
复制标题
DOI：
10.1016/j.bbrc.2008.05.149
复制
发表时间：
2008-08-15
影响因子：
3.1
通讯作者：
Furukawa，Keiko
中科院分区：
文献类型：
--
作者：
Ohkawa，Yuki;Miyazaki，Sayaka;Furukawa，Keiko
我们报道了神经节苷脂GD3在胎牛血清刺激后促进细胞增殖和黑色素瘤侵袭，导致p130Cas和paxillin的酪氨酸磷酸化增强。除了通过生长因子/受体的信号外，通过整合素的粘附信号也可能被GD3增强。本研究考察了整合素介导的信号在细胞增殖和侵袭以及适配器分子激活中的作用，表明整合素对细胞生长和侵袭也很重要。在没有血清的情况下，p130Cas和paxillin在GD3+细胞中的酪氨酸磷酸化比在GD3−细胞中的酪氨酸磷酸化更强。另一方面，在胎牛血清刺激下，悬浮状态下的GD3+和GD3 -细胞中没有蛋白发生酪氨酸磷酸化。这些结果表明，整合素介导的信号在GD3对黑色素瘤恶性特性的影响中是必不可少的。GD3和整合素在黏附病灶处的共定位支持了这些结果。
We reported that ganglioside GD3 enhances cell proliferation and invasion of melanomas causing stronger tyrosine-phosphorylation of p130Cas and paxillin after stimulation with fetal calf serum. Besides signals via growth factor/receptor, adhesion signals via integrin might be also enhanced by GD3. Here, roles of integrin-mediated signaling in the cell proliferation and invasion, and in the activation of adaptor molecules were examined, showing that integrin was also important for the cell growth and invasion. p130Cas and paxillin underwent stronger tyrosine-phosphorylation in GD3+ cells than in GD3− cells during the adhesion in the absence of serum. On the other hand, no proteins underwent tyrosine phosphorylation in GD3+ and GD3− cells in a suspension state when stimulated with fetal calf serum. These results suggested that integrin-mediated signaling is essential in the effects of GD3 on the malignant properties of melanomas. Co-localization of GD3 and integrin at the focal adhesion supported these results.