Expression Status of Serine Protease 27: A Prognostic Marker for Esophageal Squamous Cell Carcinoma Treated with Preoperative Chemotherapy/Chemoradiotherapy
Expression Status of Serine Protease 27: A Prognostic Marker for Esophageal Squamous Cell Carcinoma Treated with Preoperative Chemotherapy/Chemoradiotherapy
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DOI：
10.1245/s10434-020-09550-y
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发表时间：
2021-01-15
影响因子：
3.7
通讯作者：
Kawamura, Yuki, I
中科院分区：
文献类型：
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作者：
Kataoka, Atsuko;Yamada, Kazuhiko;Kawamura, Yuki, I
背景资料。先前的一项研究对配对的正常和食道鳞状细胞癌(ESCC)组织样本进行了转录组分析。结果表明，肿瘤组织中丝氨酸蛋白酶27(PRSS27)的表达受到干扰。因此，这项回顾性研究的目的是验证PRSS27在接受食管癌手术前治疗的患者中的预后意义。我们招募了86名食管鳞癌患者，他们在食道切除术前接受了术前治疗。采用免疫组织化学方法检测PRSS27在食管鳞癌手术切除标本和术前活检组织中的表达情况，并分析其与临床病理特征和预后的关系。在正常食道黏膜组织中，PRSS27表达于基底层和基底层的棘细胞胞浆中。在手术切除的鳞癌组织中，35例(54.7%)表达PRSS27，29例(45.3%)不表达。此外，还观察到PRSS27的异位核表达。多因素分析显示，肿瘤切除标本中PRSS27的表达是预后不良的预测指标。在活检标本中观察到PRSS27表达的病例中，PRSS27阴性的切除肿瘤患者的术后预后好于PRSS27阳性的切除肿瘤的患者。切除的ESCC组织标本中PRSS27的表达是术前治疗的ESCC患者的一个不良预后因素。此外，PRSS27在活检标本中的阳性表达转变为在切除的肿瘤标本中的阴性表达是这些患者良好预后的预测指标。因此，PRSS27状态是ESCC患者辅助治疗决策的有效工具。
Background. A previous study conducted a transcriptome analysis of paired normal and esophageal squamous cell carcinoma (ESCC) tissue samples. The results showed that the expression of serine protease 27 (PRSS27) was perturbed in tumor samples. Hence, this retrospective study aimed to validate the prognostic significance of PRSS27 in patients with preoperative treatment for ESCC.Methods. We enrolled 86 patients who received preoperative treatment before esophagectomy for ESCC. The expression of PRSS27 in resected ESCC and biopsy tissue samples obtained before preoperative treatment was evaluated via immunostaining, and its relationship with clinicopathological features and prognosis was analyzed.Results. In normal esophageal mucosa tissue samples, PRSS27 was expressed in the cytoplasm of spinous cells in the suprabasal layer and basal cells in the basal layer. Of 64 resected ESCC tissue samples, 35 (54.7%) expressed PRSS27 and 29 (45.3%) did not. Moreover, ectopic nuclear expression of PRSS27 was observed. Based on multivariate analysis, PRSS27 expression in resected tumor samples was a predictor of poor prognosis. In cases in which PRSS27 expression was observed in biopsy samples, patients with PRSS27-negative resected tumors had a better postoperative prognosis than those with PRSS27-positive resected tumors.Conclusions. PRSS27 expression in resected ESCC tissue samples is a poor prognostic factor in ESCC patients with preoperative treatment. Furthermore, conversion of PRSS27 expression from positive in biopsy samples to negative in resected tumor samples is a predictor of good prognosis in these patients. Hence, PRSS27 status is an effective tool for decision making regarding adjuvant treatment in ESCC patients.