Correlation between the genetic diversity and variation of total phenolic acids contents in Fructus Xanthii from different populations in China
Correlation between the genetic diversity and variation of total phenolic acids contents in Fructus Xanthii from different populations in China
复制标题
DOI：
10.1002/bmc.956
复制
发表时间：
2008-05-01
影响因子：
1.8
通讯作者：
Qin, Lu-Ping
中科院分区：
文献类型：
--
作者：
Han, Ting;Hu, Yuan;Qin, Lu-Ping
苍耳子是中国药典规定的用于治疗鼻部疾病和头痛的传统中药。为有效地控制苍耳属植物的药用价值，通过化学变异模式反映苍耳属植物的遗传多样性，采用紫外分光光度法、高效液相色谱指纹图谱法和ISSR分子标记技术对16个苍耳居群进行了分析。HPLC分析结果表明，苍耳属16个居群的化学成分差异较大，聚类分析结果将苍耳属16个居群分为3个化学型。苍耳居群具有丰富的遗传多样性，根据其ISSR数据可将其分为3类，并因种而异。将遗传差异和化学差异结合起来，得到了一个重要的结果，即在两种化学型中，苍耳子中总酚酸（TPA）含量较高，表明遗传多样性较大（I）。我们认为，遗传多样性影响TPA的含量。由于不同的酚酸含量可能会影响治疗效果，重要的是要指出，结合使用的遗传基础与化学型将有助于控制有利的化学型和培育新品种更理想的化学成分。版权所有（C）2008约翰威利父子有限公司
Fructus Xanthii (Cang-Er-Zi) is a traditional Chinese medicine that is used in curing nasal diseases and headache according to the Chinese Pharmacopoeia. For the effective quality control of its medicinal values, reflected by chemical variation patterns, in addition to the relationship with genetic diversity, analyses based on UV spectrophotometry, HPLC fingerprinting and inter-simple sequence repeat (ISSR) molecular markers were carried out, involving 16 Xanthium populations from different locations in China. The HPLC data showed considerable variation of chemical constituents among the 16 Xanthium populations, and they were classified to three chemotypes by hierarchical clustering analysis. Abundant genetic diversity was detected among the Xanthium populations, which were also clustered into three groups based on their ISSR data and varied according to different species. Combining the genetic divergence and chemical differences showed an important result that, in the two chemotypes, the higher contents of total phenolic acids (TPA) in Fructus Xanthii showed greater genetic diversity (I). We suggest that genetic diversity affects the contents of TPA. Since variable phenolic acid contents may affect therapeutic efficacy, it is important to point out that combining the use of genetic base with chemotype will help control the favourable chemotypes and breed new cultivars with more desirable chemical constituents. Copyright (C) 2008 John Wiley & Sons, Ltd.