Alcohol consumption alters dopamine transporter sites in Wistar-Kyoto rat brain
Alcohol consumption alters dopamine transporter sites in Wistar-Kyoto rat brain
复制标题
DOI：
10.1016/j.brainres.2005.12.009
复制
发表时间：
2006-02-16
期刊：
影响因子：
2.9
通讯作者：
Tejani-Butt, SM
中科院分区：
文献类型：
--
作者：
Jiao, X;Paré, WP;Tejani-Butt, SM
尽管动物和人类研究表明酒精戒断后多巴胺转运蛋白(DAT)位点发生了变化，但DAT在影响酒精或抑郁行为方面的作用尚未得到广泛研究。鉴于Wistar-京都(WKY)大鼠是一种假定的抑郁行为的动物模型，本研究比较了长期饮酒对WKY和Wistar(Wis)大鼠DAT部位的影响。在酒精暴露24天后，对两种菌株的大脑切片进行[H-3]-GBR12935与DAT位点结合的放射自显影分析。结果表明，在整个实验过程中，WKY大鼠比Wis大鼠摄入了更多的酒精(P<；0.001)。对离散脑区的放射自显影分析表明，与WIS大鼠相比，饮酒增加了WKY中更多脑区的DAT部位。在WKY大鼠，[H-3]-GBR12935与DAT位点的结合在杏仁核底外侧核、中央核、外侧核、下丘脑外侧核、嗅结节、尾壳核、伏隔核和黑质增加(P<；0.05)，而在下丘脑腹内侧核和海马CA1区减少。在WIS大鼠中，饮酒增加了海马区CA1区、杏仁核基底外侧核、腹侧被盖区和黑质的DAT部位，减少了外侧和腹内侧部下丘脑和齿状回的DAT部位。这些结果表明DAT部位的应变依赖性改变，这可能与饮酒后特定脑区多巴胺神经传递的改变有关。(C)2005 Elsevier B.V.保留所有权利。
Even though animal and human studies show alterations in dopamine transporter (DAT) sites after alcohol withdrawal, the role of DAT in influencing either alcoholic or depressive behavior has not been examined extensively. Given that the Wistar-Kyoto (WKY) rat is a putative animal model of depressive behavior, the present study examined the effects of chronic alcohol consumption on DAT sites in WKY versus Wistar (WIS) rats. Brains from both strains were sectioned for autoradiographic analysis of [H-3]-GBR12935 binding to DAT sites after 24 days of alcohol exposure. The results indicated that WKY rats consumed a greater amount of alcohol (P < 0.001) than WIS rats did throughout the experiment. Autoradiographic analyses of discrete brain regions indicated that alcohol consumption increased DAT sites in a greater number of brain areas in WKY compared to WIS rats. In WKY rats, the binding of [H-3]-GBR12935 to DAT sites was increased in the basolateral, central and lateral nuclei of the amygdala, lateral nucleus of the hypothalamus, olfactory tubercle, caudate-putamen, nucleus accumbens and substantia nigra (P < 0.05) and decreased in the ventromedial nucleus of the hypothalamus and the CA1 region of the hippocampus. In WIS rats, alcohol consumption increased DAT sites in the CA1 region of the hippocampus, basolateral nucleus of the amygdala, ventral tegmental area and substantia nigra, and decreased DAT sites in the lateral and ventromedial hypothalamus and dentate gyrus. These results indicate a strain dependent alteration in DAT sites which may be related to altered dopamine neurotransmission in select brain regions following alcohol consumption. (c) 2005 Elsevier B.V. All rights reserved.