Apoptosis in displaced temporomandibular joint disc with and without reduction: an immunohistochemical study
Apoptosis in displaced temporomandibular joint disc with and without reduction: an immunohistochemical study
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DOI：
10.1111/j.1600-0714.2010.00920.x
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发表时间：
2011-01-01
影响因子：
3.3
通讯作者：
Leonardi, Rosalia
中科院分区：
文献类型：
--
作者：
Loreto, Carla;Almeida, Luis Eduardo;Leonardi, Rosalia
颞下颌关节内紊乱是由于关节盘与髁状突、关节窝和关节隆起的关系异常而引起的。两种最常见的内部紊乱类型是有复位的椎间盘前移位（ADDwR）和无复位的椎间盘前移位（ADDwoR）。椎间盘移位与退行性组织变化有关。关节盘的组织学特征反映了由异常负荷引起的普遍重塑。TMJ ID和细胞凋亡之间的相关性已被证明。很少有调查已解决的作用，细胞凋亡或半胱天冬酶的活性在TMJ ID。凋亡激活过程中进行了研究，在不同地区的光盘从18例ID（ADDwR和ADDwoR）和4具尸体（对照），重点是半胱天冬酶3的表达，其激活使死亡过程不可逆转。结果显示，ADDwR和ADDwoR中caspase 3阳性细胞的比例高于对照椎间盘。免疫阳性在椎间盘区域之间也有差异;特别是，在ADDwoR切片中，标记细胞在后椎间盘附着区比前和中间带明显更多（P < 0.01）。此外，ADDwR的前（+）和中间（++）带中标记细胞的比例显着高于ADDwoR椎间盘两条带（P < 0.05）。这些数据表明程序性细胞死亡在TMJ ID进展中的重要性。
Internal derangement (ID) of the temporomandibular joint (TMJ) is due to an abnormal relationship of the articular disc to the mandibular condyle, glenoid fossa and articular eminence. The two most common types of internal derangement are anterior disc displacement with (ADDwR) and without reduction (ADDwoR). Disc displacement is associated with degenerative tissue changes. The histological features of discs from patients with TMJ ID reflect a general remodelling caused by abnormal loading. A correlation has been demonstrated between TMJ ID and apoptosis. Few investigations have addressed the role of apoptosis or caspase activity in TMJ ID. The apoptosis activation process was studied in different areas of discs from 18 patients with ID (both ADDwR and ADDwoR) and four cadavers (controls), with emphasis on the expression of caspase 3, whose activation makes the death process irreversible. The results showed a greater proportion of caspase 3-positive cells in ADDwR and ADDwoR than in control discs. Immunopositivity also varied between disc areas; in particular, in ADDwoR sections labelled cells were significantly more numerous (P < 0.01) in the posterior disc attachment than in the anterior and intermediate bands. In addition, a significantly greater proportion of labelled cells was seen in the anterior (+) and intermediate (++) band of ADDwR compared with ADDwoR discs both bands (P < 0.05). These data suggest the importance of programmed cell death in the progression of TMJ ID.