Complete Structural Elucidation of a Novel Lipooligosaccharide from the Outer Membrane of the Marine Bacterium Shewanella pacifica
Complete Structural Elucidation of a Novel Lipooligosaccharide from the Outer Membrane of the Marine Bacterium Shewanella pacifica
复制标题
海洋细菌太平洋希瓦氏菌外膜中新型脂寡糖的完整结构解析
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
M. Parrilli
中科院分区：
文献类型：
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作者：
A. Silipo;S. Leone;A. Molinaro;L. Sturiale;D. Garozzo;E. Nazarenko;R. P. Gorshkova;E. Ivanova;R. Lanzetta;M. Parrilli
太平洋希瓦氏菌（Shewanella pacifica）是一种能在海水中生长的革兰氏阴性微生物。一种新的脂寡糖（LOS）已从该细菌的外膜中分离出来，其一级结构已得到充分表征。第一次，2，3-二羟基丙酸残基（甘油酸）的存在已被确定在核心区域。通过组成和甲基化分析，通过MALDI质谱，以及通过选择性降解LOS形成的寡糖上的1H、13 C和31 P NMR光谱确定LOS的完整结构。强碱性处理，旨在恢复和确定完整的碳水化合物骨架，进行肼解，然后用热KOH脱N-酰化，而温和的肼解（脱O-酰化）使我们能够获得有关磷酸盐和其他非碳水化合物取代基的核心寡糖的性质的信息。采用温和的酸水解以获得脂质A部分，用其进行进一步的降解和质谱实验以确定其一级结构。（© Wiley-VCH Verlag GmbH & Co. KGaA，69451魏因海姆，德国，2005）
Shewanella pacifica is a Gram-negative microrganism that is able to grow in sea water. A novel lipooligosaccharide (LOS) has been isolated from the outer membrane of this bacterium and its primary structure fully characterised. For the first time, the presence of a 2,3-dihydroxypropanoic acid residue (glyceric acid) has been identified in the core region. The complete structure of the LOS was determined by compositional and methylation analyses, by MALDI mass spectrometry, and by 1H, 13C and 31P NMR spectroscopy on the oligosaccharides formed by selective degradation of the LOS. Strong alkaline treatment, aimed at recovering and identifying the complete carbohydrate backbone, was carried out by hydrazinolysis followed by de-N-acylation with hot KOH, whereas mild hydrazinolysis (de-O-acylation) allowed us to gain information about the nature of the phosphate and other non-carbohydrate substituents on the core oligosaccharide. Mild acid hydrolysis was employed to obtain a lipid A moiety with which further degradation and mass spectrometry experiments were carried out in order to determine its primary structure. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005)
电子受体和供体对腐败希瓦氏菌 MR-1 转化四氯甲烷的影响。
影响因子：
2.1
作者：
Petrovskis,EA;Vogel,TM;Adriaens,P
通讯作者：
Adriaens,P