Lentivirus mediated silencing of ubiquitin specific peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro.
Lentivirus mediated silencing of ubiquitin specific peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro.
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DOI：
10.1186/s40659-015-0006-y
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发表时间：
2015-03-19
影响因子：
6.7
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Pan Z;Pan H;Zhang J;Yang Y;Liu H;Yang Y;Huang G;Ni J;Huang J;Zhou W
泛素特异性肽酶39（USP 39）是一种65 kDa的SR相关蛋白，参与RNA剪接。以往的研究表明USP 39与人乳腺癌细胞的成瘤有关。在本研究中，我们研究了USP 39在人肝癌细胞系SMMC-7721中的功能。我们通过慢病毒介导的RNA干扰敲低USP 39的表达。qRT-PCR和western blotting检测结果表明，USP 39特异性shRNA转染SMMC-7721细胞后，其mRNA和蛋白水平均受到有效抑制。MTT法测定细胞生长受到明显抑制。结晶紫染色表明，USP 39敲低后，菌落数量和大小均减少。抑制USP 39可使SMMC-7721细胞阻滞于G2/M期。此外，膜联蛋白V显示USP 39的下调显著增加了凋亡细胞的数量。以上结果提示USP 39对肝癌细胞的增殖具有重要作用，是肝癌分子治疗的潜在靶点。本文的在线版本（doi：10.1186/s40659-015-0006-y）包含补充材料，可供授权用户使用。
Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after knock-down of USP39. Furthermore, suppression of USP39 arrested cell cycle progression at G2/M phase in SMMC-7721cells. In addition, Annexin V showed that downregulation of USP39 significantly increased the population of apoptotic cells. All our results suggest that USP39 is important for HCC cell proliferation and is a potential target for molecular therapy of HCC. The online version of this article (doi:10.1186/s40659-015-0006-y) contains supplementary material, which is available to authorized users.