GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5
GhZFP1, a novel CCCH-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with GZIRD21A and GZIPR5
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DOI：
10.1111/j.1469-8137.2009.02838.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Zheng, Cheng-Chao
中科院分区：
文献类型：
--
作者：
Guo, Ying-Hui;Yu, Yue-Ping;Zheng, Cheng-Chao
锌指蛋白是一个超家族，参与植物生长发育的许多方面。然而，对CCCH型锌指蛋白在植物抗逆性中的作用还知之甚少。利用差异杂交技术，从盐诱导的棉花(GhZFP1)cDNA文库中克隆了一个新的CCCH型锌指蛋白基因，并对其在转基因烟草中的表达进行了研究。NC89。通过酵母双杂交筛选(Y2H)，分离到与GhZFP 1相互作用的蛋白质GZIRD21A(GhZFP 1与脱水蛋白21A相互作用并对脱水蛋白21A有反应)和GZIPR5(GhZFP 1相互作用并与病程相关蛋白5相互作用)。GhZFP 1含有两个典型的锌指基序(Cx8Cx5Cx3H和Cx5Cx4Cx3H)、一个可能的核输出序列(NES)和一个潜在的核定位信号(NLS)。利用GhZFP1：：GFP融合基因在洋葱表皮细胞中的瞬时表达分析表明，GhZFP 1存在核定位。RNA印迹分析表明，GhZFP1转录本受到盐、干旱和水杨酸的诱导。通过截断突变鉴定了GhZFP1中与GZIRD21A和GZIPR5相互作用的区域，GhZFP1在转基因烟草中的过表达提高了烟草对盐胁迫的耐性和对纹枯病菌的抗性。新植物学家(2009)183：62-75doi：10.1111/j.1469-8137.2009.02838.x.
Zinc finger proteins are a superfamily involved in many aspects of plant growth and development. However, CCCH-type zinc finger proteins involved in plant stress tolerance are poorly understood.A cDNA clone designated Gossypium hirsutum zinc finger protein 1 (GhZFP1), which encodes a novel CCCH-type zinc finger protein, was isolated from a salt-induced cotton (G. hirsutum) cDNA library using differential hybridization screening and further studied in transgenic tobacco Nicotiana tabacum cv. NC89. Using yeast two-hybrid screening (Y2H), proteins GZIRD21A (GhZFP1 interacting and responsive to dehydration protein 21A) and GZIPR5 (GhZFP1 interacting and pathogenesis-related protein 5), which interacted with GhZFP1, were isolated.GhZFP1 contains two typical zinc finger motifs (Cx8Cx5Cx3H and Cx5Cx4Cx3H), a putative nuclear export sequence (NES) and a potential nuclear localization signal (NLS). Transient expression analysis using a GhZFP1::GFP fusion gene in onion epidermal cells indicated a nuclear localization for GhZFP1. RNA blot analysis showed that the GhZFP1 transcript was induced by salt (NaCl), drought and salicylic acid (SA). The regions in GhZFP1 that interact with GZIRD21A and GZIPR5 were identified using truncation mutations.Overexpression of GhZFP1 in transgenic tobacco enhanced tolerance to salt stress and resistance to Rhizoctonia solani. Therefore, it appears that GhZFP1 might be involved as an important regulator in plant responses to abiotic and biotic stresses.New Phytologist (2009) 183: 62-75doi: 10.1111/j.1469-8137.2009.02838.x.