Toward automatic reconstruction of a highly resolved tree of life
Toward automatic reconstruction of a highly resolved tree of life
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DOI：
10.1126/science.1123061
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发表时间：
2006-03-03
期刊：
影响因子：
56.9
通讯作者：
Bork, P
中科院分区：
文献类型：
--
作者：
Ciccarelli, FD;Doerks, T;Bork, P
我们已经开发了一个自动化的程序，用于重建生命树的分支长度在所有三个域可比。这棵树的基础是在191个物种中发生的31个直系同源物的串联。它揭示了域间的差异，在分类学分类。系统检测和随后排除的产品水平基因转移增加了系统发育分辨率，使我们能够确认公认的关系和解决有争议的和初步的分类。例如，我们将酸杆菌门作为变形菌门的姐妹群，支持细菌的革兰氏阳性起源，并提出嗜热的最后一个普遍的共同祖先。
We have developed an automatable procedure for reconstructing the tree of life with branch lengths comparable across all three domains. The tree has its basis in a concatenation of 31 orthologs occurring in 191 species with sequenced genomes. It revealed interdomain discrepancies in taxonomic classification. Systematic detection and subsequent exclusion of products of horizontal gene transfer increased phylogenetic resolution, allowing us to confirm accepted relationships and resolve disputed and preliminary classifications. For example, we place the phylum Acidobacteria as a sister group of delta-Proteobacteria, support a Gram-positive origin of Bacteria, and suggest a thermophilic last universal common ancestor.