A re-examination of proximodistal patterning during vertebrate limb development
A re-examination of proximodistal patterning during vertebrate limb development
复制标题
DOI：
10.1038/nature00945
复制
发表时间：
2002-08-01
期刊：
影响因子：
64.8
通讯作者：
Tabin, CJ
中科院分区：
文献类型：
--
作者：
Dudley, AT;Ros, MA;Tabin, CJ
“进步区”模型为理解脊椎动物肢体的渐进发育提供了一个框架(1)。该模型认为，在肢芽远端固定大小的区域（进展区）中，未分化的细胞经历了位置信息的渐进式变化，使得它们的规格从更近端的命运改变到更远的命运。这种位置变化被认为是由一个内部时钟驱动的，只要细胞处于进展区，这个时钟就会保持活跃。然而，由于细胞分裂，这些细胞中最近的细胞不断被推到区域的范围之外。当它们退出时，时钟功能停止，单元格被固定在区域内最后获得的位置值。与此模型相反，我们的数据表明，在肢体发育的早期，不同的肢体节段被“指定”为不同的区域，随后的发展涉及这些祖群体在分化之前的扩张。然而，我们也发现，远端肢体间充质逐渐“确定”，即不可逆地固定在逐渐有限的潜在近端命运范围内。
The 'progress zone' model provides a framework for understanding progressive development of the vertebrate limb(1). This model holds that undifferentiated cells in a zone of fixed size at the distal tip of the limb bud (the progress zone) undergo a progressive change in positional information such that their specification is altered from more proximal to more distal fates. This positional change is thought to be driven by an internal clock that is kept active as long as the cells remain in the progress zone. However, owing to cell division, the most proximal of these cells are continually pushed outside the confines of the zone. As they exit, clock function ceases and cells become fixed with the positional value last attained while within the zone. In contrast to this model, our data suggest that the various limb segments are 'specified' early in limb development as distinct domains, with subsequent development involving expansion of these progenitor populations before differentiation. We also find, however, that the distal limb mesenchyme becomes progressively 'determined', that is, irreversibly fixed, to a progressively limited range of potential proximodistal fates.