Complete Meiosis from Human Induced Pluripotent Stem Cells
Complete Meiosis from Human Induced Pluripotent Stem Cells
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DOI：
10.1002/stem.672
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发表时间：
2011-08-01
期刊：
影响因子：
5.2
通讯作者：
Belmonte, J. C. Izpisua
中科院分区：
文献类型：
--
作者：
Eguizabal, C.;Montserrat, N.;Belmonte, J. C. Izpisua
配子失败导致的不孕症影响着全世界数百万人;对于许多患者来说，无关捐赠者的配子捐赠是唯一可用的治疗方法。胚胎干细胞（ESC）可以在体外分化成生殖样细胞，但它们与患者的遗传无关。使用体外方案，旨在重演发展，我们已经实现了，第一次，人类诱导多能干细胞（hiPSC）的减数分裂后细胞的完全分化。与以前使用人胚胎干细胞的报道不同，减数分裂后的细胞在没有生殖系相关转录因子过表达的情况下出现。此外，我们一致地从不同来源（角质形成细胞和脐带血）的hiPSC中获得单倍体细胞，这些细胞由不同数量的转录因子产生，并且具有两种遗传性别，这表明我们的方法独立于重编程体细胞的表观遗传记忆。我们的工作使我们更接近于在体外生产个性化的人类配子。干细胞2011; 29：1186-1195
Gamete failure-derived infertility affects millions of people worldwide; for many patients, gamete donation by unrelated donors is the only available treatment. Embryonic stem cells (ESCs) can differentiate in vitro into germ-like cells, but they are genetically unrelated to the patient. Using an in vitro protocol that aims at recapitulating development, we have achieved, for the first time, complete differentiation of human induced pluripotent stem cells (hiPSCs) to postmeiotic cells. Unlike previous reports using human ESCs, postmeiotic cells arose without the over-expression of germline related transcription factors. Moreover, we consistently obtained haploid cells from hiPSCs of different origin (keratinocytes and cord blood), produced with a different number of transcription factors, and of both genetic sexes, suggesting the independence of our approach from the epigenetic memory of the reprogrammed somatic cells. Our work brings us closer to the production of personalized human gametes in vitro. STEM CELLS 2011; 29: 1186-1195