Prostaglandin metabolism. II. Identification of two 15-hydroxyprostaglandin dehydrogenase types.
Prostaglandin metabolism. II. Identification of two 15-hydroxyprostaglandin dehydrogenase types.
复制标题
前列腺素代谢。
DOI：
10.1016/s0021-9258(19)41931-5
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
L. Levine
中科院分区：
文献类型：
--
作者：
S. Lee;L. Levine
用放射免疫法测定几种哺乳动物组织匀浆中15-羟基前列腺素脱氢酶的活性。检测到两种酶的活性。一种是在猴的肺、心、肝、肾和脾以及鸡心和狗的肺中发现了一种比NADP-LU更有效地使用NAD-plus作为辅助因子的基因。第二种类型是在猴子、人脑、红细胞和猪肾中发现的，它使用NADP+作为辅助因子比NAD+更有效。这两种类型的15-羟基前列腺素脱氢酶是从猴脑和鸡心中部分纯化出来的。除了不同的辅因子需求外，这两种部分纯化的酶还可以通过层析性质、它们与前列腺素I2和F2pha的相对亲和力以及它们对还原的吡啶核苷酸、甲状腺激素和前列腺素B2抑制的敏感性来区分。
Homogenates of several mammalian tissues were measured by radioimmunoassay for 15-hydroxyprostaglandin dehydrogenase activity. Two types of enzyme activity were detected. One, which used NAD-plus as cofactor much more effectively than NADP-lus, was found in monkey lung, heart, liver, kidney, and spleen and in chicken heart and dog lung. A second type, which uses NADP-plus as a cofactor more effectively than NAD-plus, was found in monkey and human brain and red blood cells and in swine kidney. These two types of 15-hydroxyprostaglandin dehydrogenase were partially purified from monkey brain and chicken heart. In addition to different cofactor requirements, the two partially purified enzymes could be distinguished by chromatographic properties, their relative affinities for prostaglandin I2 and F2alpha, and their sensitivities to inhibition by reduced pyridine nucleotides, thyroid hormones, and prostaglandin B2.