Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants
Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants
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DOI：
10.1093/jb/mvj141
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发表时间：
2006-08-01
影响因子：
2.7
通讯作者：
Wada, Hajime
中科院分区：
文献类型：
--
作者：
Sakurai, Isamu;Shen, Jian-Ren;Wada, Hajime
本文分析了两种蓝藻（vulcanus Thermosynechococcus vulcanus和Synechocystis sp. PCC6803）和两种高等植物（菠菜和水稻）制备的二聚体光系统II复合物中的脂质，以确定光系统II复合物中存在多少脂质分子和哪一类脂质。据估计，每个单体分别有27,20,8和7个脂质分子与T. vulcanus, Synechocystis，菠菜和水稻的二聚体光系统Il复合物结合。在每种生物中，光系统11复合物的脂质组成与用于制备复合物的类囊体膜的脂质组成大不相同。磷脂酰甘油的含量。在光系统中，每一种生物的11个复合物远高于类囊体膜。磷脂酶A(2)处理可降解磷脂酰甘油的聚囊藻光系统11复合体导致Q(B)介导的电子传递受损，而不是Q(A)介导的电子传递受损。这些发现表明磷脂酰甘油在光系统11复合物中Q(B)结合位点的电子传递中起重要作用。
Lipids in dimeric photosystem II complexes prepared from two species of cyanobacteria, Thermosynechococcus vulcanus and Synechocystis sp. PCC6803, and two higher plants, spinach and rice, were analyzed to determine how many lipid molecules and what class of lipids are present in the photosystem Il complexes. It was estimated that 27, 20, 8, and 7 lipid molecules per monomer are bound to the dimeric photosystem Il complexes of T. vulcanus, Synechocystis, spinach, and rice, respectively. In each of the organisms, the lipid composition of the photosystem 11 complexes was quite different from that of the thylakoid membranes used for preparation of the complexes. The content of phosphatidylglycerol. in the photosystem 11 complexes of each organism was much higher than that in the thylakoid membranes. Phospholipase A(2) treatment of the photosystem 11 complexes of Synechocystis that degraded phosphatidylglycerol resulted in impairment of Q(B)-mediated but not Q(A)-mediated electron transport. These findings suggest that phosphatidylglycerol plays important roles in the electron transport at the Q(B)-binding site in photosystem 11 complexes.