TRANSCRIPTIONAL REGULATORY REGIONS OF TESTIS-SPECIFIC PGK2 DEFINED IN TRANSGENIC MICE
TRANSCRIPTIONAL REGULATORY REGIONS OF TESTIS-SPECIFIC PGK2 DEFINED IN TRANSGENIC MICE
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DOI：
10.1073/pnas.86.21.8437
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
SIMON, MI
中科院分区：
文献类型：
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作者：
ROBINSON, MO;MCCARREY, JR;SIMON, MI
编码睾丸特异性磷酸甘油酸激酶 2（PGK；ATP：3-磷酸-D-甘油酸 1-磷酸转移酶，EC 2.7.2.3）的基因仅在减数分裂和单倍体雄性生殖细胞中表达。含有 8 KB 人类基因组 PGK2 基因的转基因小鼠以组织特异性和发育调节的方式表达人类基因。为了确定控制该表达的序列的性质和位置，对具有与氯霉素乙酰转移酶(CAT)基因融合的不同长度的人PGK2 5''区域的转基因小鼠进行表达分析。发现编码区 5'' 处的 323 个碱基对区域包含 CAT 报告基因的组织特异性和发育调节表达所必需的信息。将含有 PGK2/荧光素酶编码构建体的转基因小鼠与含有等效 CAT 构建体的小鼠进行比较。荧光素酶基因表达也是睾丸特异性的，并且比 CAT 基因表达更敏感，但在转基因小鼠的生殖细胞中，两个报告基因的其他调节是相似的。 PGK2/CAT 和 PGK2/荧光素酶融合基因的翻译与第一个可检测的转录本同时发生。
The gene encoding testis-specific phosphoglycerate kinase 2 (PGK; ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) is expressed only in meiotic and haploid male germ cells. Transgenic mice containing an 8-kilobase human genomic PGK2 gene express the human gene in a tissue-specific and developmentally regulated manner. To determine the nature and lcoation of sequences controlling this expression, transgenic mice with various lengths of the human PGK2 5'' region fused to the chloramphenicol acetyltransferase (CAT) gene were anlayzed for expression. A 323-base-pair region 5'' to the coding region was found to contain information essential for both tissue-specific and developmentally regulated expression of the CAT reporter gene. Transgenic mice containing a PGK2/luciferase-coding construct were compared with mice containing an equivalent CAT construct. Luciferase gene expression was also testis-specific and was more sensitive than CAT gene expression, but otherwise regulation of the two reporter genes was similar in the germ cells of transgenic mice. Translation of both PGK2/CAT and PGK2/luciferase fusion genes was seen concurrently with the first detectable transcripts.