Expression of interleukin-8 correlates with vascularity in human gastric carcinomas.
Expression of interleukin-8 correlates with vascularity in human gastric carcinomas.
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发表时间：
1998
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通讯作者：
Y. Kitadai;K. Haruma;K. Sumii;Soichiro Yamamoto;T. Ue;H. Yokozaki;Wataru Yasui;Yasukazu Ohmoto;G. Kajiyama;I. J. Fidler;Eiichi Tahara
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作者：
Y. Kitadai;K. Haruma;K. Sumii;Soichiro Yamamoto;T. Ue;H. Yokozaki;Wataru Yasui;Yasukazu Ohmoto;G. Kajiyama;I. J. Fidler;Eiichi Tahara
白细胞介素（IL）-8是一种多功能的细胞因子，可以刺激内皮细胞的分裂。应用北方杂交和原位杂交技术检测了8株人胃癌细胞系、39例胃癌组织及相应的正常胃粘膜（34例手术标本和5例活检标本）中IL-8 mRNA的表达，并应用酶联免疫吸附试验和免疫组化技术检测了IL-8蛋白的表达。在8株人胃癌细胞系中，6株表达1.8kb IL-8 mRNA，并分泌不同水平的IL-8蛋白。通过暴露于IL-1 α、表皮生长因子和转化生长因子-α诱导TMK-1细胞表达IL-8，这些因子先前被证明是人胃癌细胞的自分泌生长刺激因子。在肿瘤组织中，大多数肿瘤（34例手术标本中的28例和5例活检标本中的4例）的IL-8表达水平高于相应的正常粘膜。原位杂交和免疫组化结果显示，IL-8 mRNA和蛋白定位于肿瘤细胞的胞浆中。用抗CD 34抗体检测胃癌组织中的血管数目。肿瘤组织中IL-8 mRNA水平与血管生成密切相关（斯皮尔曼相关，r = 0.812; P = 0.001）。这些数据表明，肿瘤细胞产生的IL-8可以调节新生血管，从而调节人胃癌的生长和扩散。
Interleukin (IL)-8 is a multifunctional cytokine that can stimulate the division of endothelial cells. We examined the expression of IL-8 mRNA using Northern blot analysis and in situ mRNA hybridization (ISH) and protein production using enzyme-linked immunosorbent assay and immunohistochemistry in 8 human gastric carcinoma cell lines and 39 gastric carcinomas and corresponding normal mucosa (34 surgical specimens and 5 biopsy specimens). Of the 8 human gastric carcinoma cell lines, 6 expressed 1.8-kb IL-8 mRNA and secreted various levels of IL-8 protein. The expression of IL-8 by TMK-1 cells was induced by exposure to IL-1 alpha, epidermal growth factor, and transforming growth factor-alpha, shown previously to be autocrine growth stimulators for human gastric carcinoma cells. In tumor tissues, most of the tumors (28 of 34 surgical specimens and 4 of 5 biopsy specimens) expressed IL-8 at higher levels than the corresponding normal mucosa. ISH and immunohistochemical analyses revealed that IL-8 mRNA and protein were localized in the cytoplasm of tumor cells. The number of blood vessels in the gastric carcinomas was determined by using antibodies against CD34. The level of IL-8 mRNA in the neoplasms strongly correlated with vascularization (Spearman correlation, r = 0.812; P = 0.001). The data suggest that IL-8 produced by tumor cells may regulate neovascularization and, hence, the growth and spread of human gastric carcinoma.