LUNG DEFORMATION AND MACROPHAGE DISPLACEMENT IN SMOKE-EXPOSED AND NORMAL MICE (MUS-MUSCULUS) FOLLOWING DIFFERENT FIXATION PROCEDURES
LUNG DEFORMATION AND MACROPHAGE DISPLACEMENT IN SMOKE-EXPOSED AND NORMAL MICE (MUS-MUSCULUS) FOLLOWING DIFFERENT FIXATION PROCEDURES
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DOI：
10.1007/bf00710905
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
MATULIONIS, DH
中科院分区：
文献类型：
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作者：
MATULIONIS, DH
在香烟烟雾暴露、假处理和对照苍白雄性小鼠中，在浸泡固定、肺内滴注固定剂和肺灌洗之后，评价肺变形（收缩或膨胀）和肺实质巨噬细胞的移位。肺体积位移和肺切片和肺泡面积分析显示，通过浸泡固定的所有治疗组的肺变形程度一致，但通过灌注固定剂和灌洗后灌注固定剂的肺变形程度不同。在所有相应的治疗组中，固定液滴注肺和固定液滴注后灌洗肺的单位肺截面积的原位肺实质巨噬细胞数量显著大于浸泡固定后的数量。在固定剂灌注和灌洗后灌注固定剂肺的气道中观察到缺乏巨噬细胞。浸泡固定后，肺巨噬细胞均匀分布在整个肺实质中，而在滴入固定剂肺之前滴入固定剂和灌洗的情况下，这些细胞往往集中在末端细支气管附近的肺泡中。灌洗程序去除了未知部分的肺巨噬细胞，似乎无法有效地对肺实质巨噬细胞群进行采样。气管内滴注固定剂（有或没有预先灌洗）通过将气道吞噬细胞转移到肺泡中，明显改变了肺巨噬细胞的分布。报告的数据表明，原位肺实质巨噬细胞群的分数估计值可通过对浸没固定的肺组织中每单位面积的这些细胞数量进行计数来获得。
Lung deformation (shrinkage or inflation) and displacement of pulmonary parenchymal macrophages were evaluated after immersion fixation, intratracheal instillation of fixative and lung lavage followed by intratracheal fixative instillation in cigarette smoke-exposed, sham-treated and control pallid male mice. Lung volume displacement and lung section and alveolar area analysis revealed that degree of deformation was uniform in lungs from all treatment groups fixed by immersion but not by instillation of fixative and fixative instillation following lavage. In situ pulmonary parenchymal macrophage number per lung section area of fixative-instilled lungs and lavaged lungs followed by fixative instillation was significantly greater than in those following immersion fixation in all corresponding treatment groups. A paucity of macrophages was noted in airways of fixative-instilled and lavaged followed by instillation of fixative lungs. Pulmonary macrophages were uniformly distributed throughout lung parenchyma following immersion fixation, while in fixative-instilled and lavaged prior to instillation of fixative lungs these cells tended to be concentrated in alveoli near terminal bronchioles. Lavage procedures removed an unknown portion of lung macrophages and appeared to ineffectively sample the pulmonary parenchymal macrophage population. Intratracheal instillation of fixative with or without prior lavage apparently alters the distribution of pulmonary macrophages by displacing airway phagocytes into the alveoli. Data reported suggest that fractional estimates of in situ lung parenchymal macrophage population can be obtained by counting the number of these cells per area of tissue from lungs fixed by immersion.