Effects of dried bonito (Katsuobushi) and captopril, an angiotensin I-converting enzyme inhibitor, on rat isolated aorta:: A possible mechanism of antihypertensive action
Effects of dried bonito (Katsuobushi) and captopril, an angiotensin I-converting enzyme inhibitor, on rat isolated aorta:: A possible mechanism of antihypertensive action
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DOI：
10.1271/bbb.69.911
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发表时间：
2005-05-01
影响因子：
1.6
通讯作者：
Hatae, K
中科院分区：
文献类型：
--
作者：
Kouno, K;Hirano, S;Hatae, K
为了阐明干鲣鱼（katsuobushi）的抗高血压作用的机制，我们比较了干鲣鱼提取物与血管紧张素I转换酶（ACE）抑制剂captopril对大鼠主动脉的作用。干鲣鱼提取物（3 × 10（-4）至3 × 10（-3）g/ml）对去甲肾上腺素（10（-7）M）诱导的收缩的舒张作用强于KCl（55.9 mM）诱导的收缩。干鲣鱼提取物（3 × 10（-3）g/ml）轻微抑制10（-7）m血管紧张素I诱导的收缩。与此相反，卡托普利（10（-8）至10（-7）M）不影响10（-7）M去甲肾上腺素或55.0 mM KCl诱导的收缩，但较高浓度的卡托普利（10（-6）M）可使其轻微舒张，卡托普利（10（-8）至10（-6）M）以浓度依赖性方式显著抑制10（-7）M血管紧张素I诱导的收缩。这些结果表明，干鲣鱼诱导的抗高血压作用机制涉及直接作用于血管平滑肌，除了ACE抑制活性。
In order to elucidate the mechanism of the antihypertensive action of dried bonito (katsuobushi), we compared the effects of dried bonito extracts with those of captopril, an angiotensin I-converting enzyme (ACE) inhibitor, on aorta preparations isolated from rats. Dried bonito extracts (3 x 10(-4) to 3 x 10(-3) g/ml) more potently relaxed contractions induced by norepinephrine (10(-7) M) than contractions induced by KCl (55.9 mM). Dried bonito extracts (3 x 10(-3)g/ml) slightly inhibited 10(-7) m angiotensin I-induced contractions. In contrast, captopril (10(-8) to 10(-7) m) did not affect 10(-7) M norepinephrine- or 55.0 mM KCl-induced contractions, but a higher concentration of captopril (10(-6) M) very slightly relaxed it. Captopril (10(-8) to 10(-6) M) markedly inhibited 10(-7) m angiotensin I-induced contractions in a concentration-dependent manner. These results suggest that antihypertensive mechanism of action induced by dried bonito involves direct action on vascular smooth muscle in addition to ACE-inhibitory activity.